caa a22 4500 606212892 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10295-015-1645-2 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1645-2 Two-step production of gamma-aminobutyric acid from cassava powder using Corynebacterium glutamicum and Lactobacillus plantarum [Elektronische Daten] [Taowei Yang, Zhiming Rao, Bernard Kimani, Meijuan Xu, Xian Zhang, Shang-Tian Yang] Production of gamma-aminobutyric acid (GABA) from crop biomass such as cassava in high concentration is desirable, but difficult to achieve. A safe biotechnological route was investigated to produce GABA from cassava powder by C. glutamicum G01 and L. plantarum GB01-21. Liquefied cassava powder was first transformed to glutamic acid by simultaneous saccharification and fermentation with C. glutamicum G01, followed by biotransformation of glutamic acid to GABA with resting cells of L. plantarum GB01-21 in the reaction medium. After optimizing the reaction conditions, the maximum concentration of GABA reached 80.5g/L with a GABA productivity of 2.68g/L/h. This is the highest yield ever reported of GABA production from cassava-derived glucose. The bioprocess provides the added advantage of employing nonpathogenic microorganisms, C. glutamicum and L. plantarum, in microbial production of GABA from cassava biomass, which can be used in the food and pharmaceutical industries. Society for Industrial Microbiology and Biotechnology, 2015 Gamma-aminobutyric acid nationallicence Cassava nationallicence Corynebacterium glutamicum nationallicence Lactobacillus plantarum nationallicence Yang Taowei The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut Rao Zhiming The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut Kimani Bernard The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut Xu Meijuan The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut Zhang Xian The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut Yang Shang-Tian Department of Chemical and Biomolecular Engineering, The Ohio State University, 43210, Columbus, OH, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/8(2015-08-01), 1157-1165 1367-5435 42:8<1157 2015 42 10295 https://doi.org/10.1007/s10295-015-1645-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1645-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Taowei The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut NATIONALLICENCE 700 1- Rao Zhiming The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut NATIONALLICENCE 700 1- Kimani Bernard The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut NATIONALLICENCE 700 1- Xu Meijuan The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut NATIONALLICENCE 700 1- Zhang Xian The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, China aut NATIONALLICENCE 700 1- Yang Shang-Tian Department of Chemical and Biomolecular Engineering, The Ohio State University, 43210, Columbus, OH, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/8(2015-08-01), 1157-1165 1367-5435 42:8<1157 2015 42 10295