caa a22 4500 606212930 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10295-015-1631-8 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1631-8 Bioconversion of d -glucose to d -psicose with immobilized d -xylose isomerase and d -psicose 3-epimerase on Saccharomyces cerevisiae spores [Elektronische Daten] [Zijie Li, Yi Li, Shenglin Duan, Jia Liu, Peng Yuan, Hideki Nakanishi, Xiao-Dong Gao] Saccharomyces cerevisiae spores are dormant cells, which can tolerate various types of environmental stress. In our previous work, we successfully developed biological and chemical methods for enzyme immobilization based on the structures of S. cerevisiae spore wall. In this study, we employed biological and chemical approaches for the immobilization of d-xylose isomerase (XI) from Thermus thermophilus and d-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens with yeast spores, respectively. The enzymatic properties of both immobilized XI and DPEase were characterized and the immobilized enzymes exhibit higher thermostability, broader pH tolerance, and good repeatability compared with free enzymes. Furthermore, we established a two-step approach for the bioconversion of d-glucose to d-psicose using immobilized enzymes. To improve the conversion yield, a multi-pot strategy was adopted for d-psicose production by repeating the two-step process continually. As a result, the yield of d-psicose was obviously improved and the highest yield reached about 12.0%. Society for Industrial Microbiology and Biotechnology, 2015 S. cerevisiae spore nationallicence d -Xylose isomerase nationallicence d -Psicose 3-epimerase nationallicence Enzyme immobilization nationallicence d -Psicose synthesis nationallicence Li Zijie Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China aut Li Yi Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China aut Duan Shenglin China National Research Institute of Food and Fermentation Industries, 10015, Beijing, China aut Liu Jia China National Research Institute of Food and Fermentation Industries, 10015, Beijing, China aut Yuan Peng China National Research Institute of Food and Fermentation Industries, 10015, Beijing, China aut Nakanishi Hideki Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China aut Gao Xiao-Dong Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/8(2015-08-01), 1117-1128 1367-5435 42:8<1117 2015 42 10295 https://doi.org/10.1007/s10295-015-1631-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1631-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Zijie Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Li Yi Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Duan Shenglin China National Research Institute of Food and Fermentation Industries, 10015, Beijing, China aut NATIONALLICENCE 700 1- Liu Jia China National Research Institute of Food and Fermentation Industries, 10015, Beijing, China aut NATIONALLICENCE 700 1- Yuan Peng China National Research Institute of Food and Fermentation Industries, 10015, Beijing, China aut NATIONALLICENCE 700 1- Nakanishi Hideki Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Gao Xiao-Dong Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/8(2015-08-01), 1117-1128 1367-5435 42:8<1117 2015 42 10295