caa a22 4500 606212949 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-015-1602-0 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1602-0 Mussel-inspired surface modification of magneticgraphite nanosheets composite for efficient Candida rugosa lipase immobilization [Elektronische Daten] [Chen Hou, Lincheng Zhou, Hao Zhu, Xinyu Wang, Niran Hu, Fang Zeng, Liyuan Wang, Hang Yin] By the facile adhesion way, the novel composite complex by polydopamine (PDA) and magnetic graphite nanosheets (Fe3O4@GNSs) has been successfully synthesized. The resulting composite was characterized by means of scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, and Raman spectra, X-ray diffraction, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. Meanwhile, the PDA functionalized Fe3O4@GNSs (Fe3O4@GNSs-PDA) was applied for Candida rugosa lipase (CRL) immobilization covalently without any toxic coupling agent. Combining the superior physical properties and chemical stability of Fe3O4@GNSs and the well biocompatibility, functional characteristics of PDA, the Fe3O4@GNSs-PDA composite displayed several advantages, including the high enzyme capacity, enzyme activity and stability and a decrease in enzyme loss. Our work demonstrated that the mussel-inspired Fe3O4@GNSs can be extended to many other applications such as biocatalytic, genetic and industrial. Society for Industrial Microbiology and Biotechnology, 2015 Magnetic composite nationallicence Polydopamine nationallicence Enzyme immobilization nationallicence Enzyme activity nationallicence Thermo-stability nationallicence Hou Chen State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut Zhou Lincheng State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut Zhu Hao State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut Wang Xinyu State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut Hu Niran State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut Zeng Fang State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut Wang Liyuan State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut Yin Hang State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 723-734 1367-5435 42:5<723 2015 42 10295 https://doi.org/10.1007/s10295-015-1602-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1602-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hou Chen State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Zhou Lincheng State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Zhu Hao State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Wang Xinyu State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Hu Niran State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Zeng Fang State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Wang Liyuan State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Yin Hang State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 723-734 1367-5435 42:5<723 2015 42 10295