caa a22 4500 606212957 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-015-1600-2 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1600-2 Temperature effects on biomass, geosmin, and 2-methylisoborneol production and cellular activity by Nocardia spp. and Streptomyces spp. isolated from rainbow trout recirculating aquaculture systems [Elektronische Daten] [Kevin Schrader, Marcuslene Harries, Phaedra Page] Isolates of Nocardia cummidelens, Nocard ia fluminea, Streptomyces albidoflavus, and Streptomyces luridiscabiei attributed as the cause of "earthy-musty” off-flavor in rainbow trout (Oncorhynchus mykiss) raised in recirculating aquaculture systems (RAS) were evaluated for the effect of temperature (10-30°C) on biomass, geosmin, and 2-methylisoborneol (MIB) production and cellular activity. Cultures of these isolates were monitored over 7days by measuring culture dry weight, geosmin, and MIB production using solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS), and ATP production via a luminometer. Compared to the other isolates, S. luridiscabiei had significantly (P<0.05) higher biomass (8.17±0.35mg/mL) at 15°C (water temperature in the RAS) after 7days incubation. In addition, S. luridiscabiei produced significantly (P<0.05) higher geosmin (69,976±15,733ng/L) at 15°C. At 25°C and 30°C, S. albidoflavus produced significantly (P<0.05) higher geosmin (182,074±60,272ng/L and 399,991±102,262ng/L, respectively). All isolates produced MIB at 15°C, but S. luridiscabiei produced significantly (P<0.05) higher MIB (97,143±28,972ng/L) and ATP after 7days. Therefore, S. luridiscabiei appears to be a likely contributor of geosmin and MIB in the RAS. Springer-Verlag (outside the USA), 2015 Aquaculture nationallicence Geosmin nationallicence 2-methylisoborneol nationallicence Nocardia nationallicence Streptomyces nationallicence Schrader Kevin United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, National Center for Natural Products Research, Post Office Box 1848, 38677-1848, University, MS, USA aut Harries Marcuslene United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, National Center for Natural Products Research, Post Office Box 1848, 38677-1848, University, MS, USA aut Page Phaedra United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, National Center for Natural Products Research, Post Office Box 1848, 38677-1848, University, MS, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 759-767 1367-5435 42:5<759 2015 42 10295 https://doi.org/10.1007/s10295-015-1600-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1600-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schrader Kevin United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, National Center for Natural Products Research, Post Office Box 1848, 38677-1848, University, MS, USA aut NATIONALLICENCE 700 1- Harries Marcuslene United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, National Center for Natural Products Research, Post Office Box 1848, 38677-1848, University, MS, USA aut NATIONALLICENCE 700 1- Page Phaedra United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, National Center for Natural Products Research, Post Office Box 1848, 38677-1848, University, MS, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 759-767 1367-5435 42:5<759 2015 42 10295