caa a22 4500 606212965 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-015-1597-6 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1597-6 New lager yeast strains generated by interspecific hybridization [Elektronische Daten] [Kristoffer Krogerus, Frederico Magalhães, Virve Vidgren, Brian Gibson] The interspecific hybrid Saccharomyces pastorianus is the most commonly used yeast in brewery fermentations worldwide. Here, we generated de novo lager yeast hybrids by mating a domesticated and strongly flocculent Saccharomyces cerevisiae ale strain with the Saccharomyces eubayanus type strain. The hybrids were characterized with respect to the parent strains in a wort fermentation performed at temperatures typical for lager brewing (12°C). The resulting beers were analysed for sugar and aroma compounds, while the yeasts were tested for their flocculation ability and α-glucoside transport capability. These hybrids inherited beneficial properties from both parent strains (cryotolerance, maltotriose utilization and strong flocculation) and showed apparent hybrid vigour, fermenting faster and producing beer with higher alcohol content (5.6 vs 4.5% ABV) than the parents. Results suggest that interspecific hybridization is suitable for production of novel non-GM lager yeast strains with unique properties and will help in elucidating the evolutionary history of industrial lager yeast. The Author(s), 2015 Lager yeast nationallicence S. eubayanus nationallicence Brewing nationallicence Hybrid nationallicence Mating nationallicence Heterosis nationallicence Krogerus Kristoffer VTT Technical Research Centre of Finland, Tietotie 2, P.O. Box 1000, 02044, Espoo, Finland aut Magalhães Frederico VTT Technical Research Centre of Finland, Tietotie 2, P.O. Box 1000, 02044, Espoo, Finland aut Vidgren Virve VTT Technical Research Centre of Finland, Tietotie 2, P.O. Box 1000, 02044, Espoo, Finland aut Gibson Brian VTT Technical Research Centre of Finland, Tietotie 2, P.O. Box 1000, 02044, Espoo, Finland aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 769-778 1367-5435 42:5<769 2015 42 10295 https://doi.org/10.1007/s10295-015-1597-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1597-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krogerus Kristoffer VTT Technical Research Centre of Finland, Tietotie 2, P.O. Box 1000, 02044, Espoo, Finland aut NATIONALLICENCE 700 1- Magalhães Frederico VTT Technical Research Centre of Finland, Tietotie 2, P.O. Box 1000, 02044, Espoo, Finland aut NATIONALLICENCE 700 1- Vidgren Virve VTT Technical Research Centre of Finland, Tietotie 2, P.O. Box 1000, 02044, Espoo, Finland aut NATIONALLICENCE 700 1- Gibson Brian VTT Technical Research Centre of Finland, Tietotie 2, P.O. Box 1000, 02044, Espoo, Finland aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 769-778 1367-5435 42:5<769 2015 42 10295