caa a22 4500 606212973 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-015-1596-7 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1596-7 Triacetic acid lactone production in industrial Saccharomyces yeast strains [Elektronische Daten] [Lauren Saunders, Michael Bowman, Jeffrey Mertens, Nancy Da Silva, Ronald Hector] Triacetic acid lactone (TAL) is a potential platform chemical that can be produced in yeast. To evaluate the potential for industrial yeast strains to produce TAL, the g2ps1 gene encoding 2-pyrone synthase was transformed into 13 industrial yeast strains of varied genetic background. TAL production varied 63-fold between strains when compared in batch culture with glucose. Ethanol, acetate, and glycerol were also tested as potential carbon sources. Batch cultures with ethanol medium produced the highest titers. Therefore, fed-batch cultivation with ethanol feed was assayed for TAL production in bioreactors, producing our highest TAL titer, 5.2g/L. Higher feed rates resulted in a loss of TAL and subsequent production of additional TAL side products. Finally, TAL efflux was measured and TAL is actively exported from S. cerevisiae cells. Percent yield for all strains was low, indicating that further metabolic engineering of the strains is required. Springer-Verlag (outside the USA), 2015 Triacetic acid lactone nationallicence Industrial yeast nationallicence Saccharomyces cerevisiae nationallicence Polyketide synthase nationallicence 2-Pyrone synthase nationallicence Saunders Lauren Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL, USA aut Bowman Michael Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL, USA aut Mertens Jeffrey Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL, USA aut Da Silva Nancy Department of Chemical Engineering and Materials Science, University of California, 92697, Irvine, CA, USA aut Hector Ronald Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 711-721 1367-5435 42:5<711 2015 42 10295 https://doi.org/10.1007/s10295-015-1596-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1596-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saunders Lauren Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Bowman Michael Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Mertens Jeffrey Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Da Silva Nancy Department of Chemical Engineering and Materials Science, University of California, 92697, Irvine, CA, USA aut NATIONALLICENCE 700 1- Hector Ronald Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 711-721 1367-5435 42:5<711 2015 42 10295