caa a22 4500 606213023 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-015-1594-9 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1594-9 Production of drug metabolites by immobilised Cunninghamella elegans [Elektronische Daten] from screening to scale up [Laura Quinn, Rita Dempsey, Eoin Casey, Ayla Kane, Cormac Murphy] Cunninghamella elegans is a fungus that has been used extensively as a microbial model of mammalian drug metabolism, whilst its potential as a biocatalyst for the preparative production of human drug metabolites has been often proposed, little effort has been made to enable this. Here, we describe a workflow for the application of C. elegans for the production of drug metabolites, starting from well-plate screening assays leading to the preparative production of drug metabolites using fungus immobilised either in alginate or as a biofilm. Using 12- and 96-well plates, the simultaneous screening of several drug biotransformations was achieved. To scale up the biotransformation, both modes of immobilisation enabled semi-continuous production of hydroxylated drug metabolites through repeated addition of drug and rejuvenation of the fungus. It was possible to improve the productivity in the biofilm culture for the production of 4′-hydroxydiclofenac from 1mg/lh to over 4mg/lh by reducing the incubation time for biotransformation and the number of rejuvenation steps. Society for Industrial Microbiology and Biotechnology, 2015 Biotransformation nationallicence Biocatalysis nationallicence Biofilm nationallicence Immobilisation nationallicence Quinn Laura School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland aut Dempsey Rita School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland aut Casey Eoin School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Ireland aut Kane Ayla School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland aut Murphy Cormac School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 799-806 1367-5435 42:5<799 2015 42 10295 https://doi.org/10.1007/s10295-015-1594-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1594-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Quinn Laura School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland aut NATIONALLICENCE 700 1- Dempsey Rita School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland aut NATIONALLICENCE 700 1- Casey Eoin School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Ireland aut NATIONALLICENCE 700 1- Kane Ayla School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland aut NATIONALLICENCE 700 1- Murphy Cormac School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 799-806 1367-5435 42:5<799 2015 42 10295