caa a22 4500 60621304X CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-015-1590-0 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1590-0 Organic acids from lignocellulose: Candida lignohabitans as a new microbial cell factory [Elektronische Daten] [Martina Bellasio, Diethard Mattanovich, Michael Sauer, Hans Marx] Biorefinery applications require microbial cell factories for the conversion of various sugars derived from lignocellulosic material into value-added chemicals. Here, the capabilities of the yeast Candida lignohabitans to utilize a range of such sugars is characterized. Substrates efficiently converted by this yeast include the pentoses xylose and arabinose. Genetic engineering of C. lignohabitans with the isolated endogenous GAP promoter and GAP terminator was successful. GFP expression was used as a proof of functionality for the isolated transcription elements. Expression of lactate dehydrogenase and cis-aconitate decarboxylase resulted in stable and reproducible production of lactic acid and itaconic acid, respectively. The desired organic acids were accumulated converting pure sugars as well as lignocellulosic hydrolysates. C. lignohabitans proved therefore to be a promising reliable microbial host for production of organic acids from lignocellulosic material. Society for Industrial Microbiology and Biotechnology, 2015 Microbial cell factory nationallicence Candida lignohabitans nationallicence Lactic acid production nationallicence Itaconic acid production nationallicence Biorefinery nationallicence Bellasio Martina Department of Biotechnology, BOKU—VIBT University of Natural Resources and Life Sciences, Muthgasse 107, 1190, Vienna, Austria aut Mattanovich Diethard Department of Biotechnology, BOKU—VIBT University of Natural Resources and Life Sciences, Muthgasse 107, 1190, Vienna, Austria aut Sauer Michael Department of Biotechnology, BOKU—VIBT University of Natural Resources and Life Sciences, Muthgasse 107, 1190, Vienna, Austria aut Marx Hans Department of Biotechnology, BOKU—VIBT University of Natural Resources and Life Sciences, Muthgasse 107, 1190, Vienna, Austria aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 681-691 1367-5435 42:5<681 2015 42 10295 https://doi.org/10.1007/s10295-015-1590-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1590-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bellasio Martina Department of Biotechnology, BOKU—VIBT University of Natural Resources and Life Sciences, Muthgasse 107, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Mattanovich Diethard Department of Biotechnology, BOKU—VIBT University of Natural Resources and Life Sciences, Muthgasse 107, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Sauer Michael Department of Biotechnology, BOKU—VIBT University of Natural Resources and Life Sciences, Muthgasse 107, 1190, Vienna, Austria aut NATIONALLICENCE 700 1- Marx Hans Department of Biotechnology, BOKU—VIBT University of Natural Resources and Life Sciences, Muthgasse 107, 1190, Vienna, Austria aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 681-691 1367-5435 42:5<681 2015 42 10295