caa a22 4500 606213066 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-014-1564-7 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1564-7 Lipid accumulation by Rhodococcus rhodochrous grown on glucose [Elektronische Daten] [Sara Shields-Menard, Marta Amirsadeghi, Badamkhand Sukhbaatar, Emmanuel Revellame, Rafael Hernandez, Janet Donaldson, W. French] Biodiesel is an alternative fuel made from costly vegetable oil feedstocks. Some microorganisms can accumulate lipids when nutrients are limited and carbon is in excess. Rhodococcus rhodochrous is a gram-positive bacterium most often used in bioremediation or acrylamide production. The purpose of this study was to investigate and characterize the lipid accumulation capabilities of R. rhodochrous. Shake flasks and a large-scale fermentation were used to cultivate R. rhodochrous in varying concentrations of glucose. R. rhodochrous achieved almost 50% of dry cell mass as lipid when grown in 20g/L of glucose. Wax esters and triglycerides were identified in R. rhodochrous lipid extract. The transesterified extractables of R. rhodochrous consisted of mostly palmitic (35%) and oleic (42%) acid methyl esters. This study shows R. rhodochrous to be an oleaginous bacterium with potential for application in alternative fuels. Society for Industrial Microbiology and Biotechnology, 2015 Rhodococcus nationallicence Lipids nationallicence Oleaginous microbes nationallicence FAMEs nationallicence Shields-Menard Sara Department of Biological Sciences, Mississippi State University, Mississippi State, MS, USA aut Amirsadeghi Marta Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, USA aut Sukhbaatar Badamkhand Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, USA aut Revellame Emmanuel Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA, USA aut Hernandez Rafael Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA, USA aut Donaldson Janet Department of Biological Sciences, Mississippi State University, Mississippi State, MS, USA aut French W. Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 693-699 1367-5435 42:5<693 2015 42 10295 https://doi.org/10.1007/s10295-014-1564-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1564-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shields-Menard Sara Department of Biological Sciences, Mississippi State University, Mississippi State, MS, USA aut NATIONALLICENCE 700 1- Amirsadeghi Marta Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, USA aut NATIONALLICENCE 700 1- Sukhbaatar Badamkhand Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, USA aut NATIONALLICENCE 700 1- Revellame Emmanuel Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA, USA aut NATIONALLICENCE 700 1- Hernandez Rafael Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA, USA aut NATIONALLICENCE 700 1- Donaldson Janet Department of Biological Sciences, Mississippi State University, Mississippi State, MS, USA aut NATIONALLICENCE 700 1- French W. Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 693-699 1367-5435 42:5<693 2015 42 10295