caa a22 4500 606213074 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-014-1578-1 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1578-1 Construction of allitol synthesis pathway by multi-enzyme coexpression in Escherichia coli and its application in allitol production [Elektronische Daten] [Yueming Zhu, Hongyi Li, Pingping Liu, Jiangang Yang, Xueli Zhang, Yuanxia Sun] An engineered strain for the conversion of d-fructose to allitol was developed by constructing a multi-enzyme coupling pathway and cofactor recycling system in Escherichia coli. d-Psicose-3-epimerase from Ruminococcus sp. and ribitol dehydrogenase from Klebsiella oxytoca were coexpressed to form the multi-enzyme coupling pathway for allitol production. The cofactor recycling system was constructed using the formate dehydrogenase gene from Candida methylica for continuous NADH supply. The recombinant strain produced 10.62g/l allitol from 100mM d-fructose. To increase the intracellular concentration of the substrate, the glucose/fructose facilitator gene from Zymomonas mobilis was incorporated into the engineered strain. The results showed that the allitol yield was enhanced significantly to 16.53g/l with a conversion rate of 92%. Through optimizing conversion conditions, allitol was produced effectively on a large scale by the whole-cell biotransformation system; the yield reached 48.62g/l when 500mM d-fructose was used as the substrate. Society for Industrial Microbiology and Biotechnology, 2015 Allitol nationallicence Multi-enzyme coupling pathway nationallicence Cofactor recycling system nationallicence Whole-cell biotransformation nationallicence Zhu Yueming National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut Li Hongyi Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut Liu Pingping National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut Yang Jiangang National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut Zhang Xueli National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut Sun Yuanxia National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 661-669 1367-5435 42:5<661 2015 42 10295 https://doi.org/10.1007/s10295-014-1578-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1578-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Yueming National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Li Hongyi Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, 030001, Taiyuan, Shanxi, China aut NATIONALLICENCE 700 1- Liu Pingping National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Yang Jiangang National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xueli National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Sun Yuanxia National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xiqi Road 32#, Tianjin Airport Economic Area, 300308, Tianjin, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 661-669 1367-5435 42:5<661 2015 42 10295