caa a22 4500 606213082 CHVBK 20210128101045.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10295-015-1593-x doi (NATIONALLICENCE)springer-10.1007/s10295-015-1593-x Rational engineering of multiple module pathways for the production of l -phenylalanine in Corynebacterium glutamicum [Elektronische Daten] [Chuanzhi Zhang, Junli Zhang, Zhen Kang, Guocheng Du, Jian Chen] Microbial production of l-phenylalanine (l-Phe) from renewable sources has attracted much attention recently. In the present study, Corynebacterium glutamicum 13032 was rationally engineered to produce l-Phe from inexpensive glucose. First, all the l-Phe biosynthesis pathway genes were investigated and the results demonstrated that in addition to AroF and PheA, the native PpsA, TktA, AroE and AroA, and the heterologous AroL and TyrB were also the key enzymes for L-Phe biosynthesis. Through combinational expression of these key enzymes, the l-Phe production was increased to 6.33±0.13gl−1 which was about 1.48-fold of that of the parent strain C. glutamicum (pXM-pheA fbr-aroF fbr) (fbr, feedback-inhibition resistance). Furthermore, the production of l-Phe was improved to 9.14±0.21gl−1 by modifying the glucose and l-Phe transport systems and blocking the acetate and lactate biosynthesis pathways. Eventually, the titer of l-Phe was enhanced to 15.76±0.23gl−1 with a fed-batch fermentation strategy. To the best of our knowledge, this was the highest value reported in rationally engineered C. glutamicum 13032 strains. The results obtained will also contribute to rational engineering of C. glutamicum for production of other valuable aromatic compounds. Society for Industrial Microbiology and Biotechnology, 2015 Corynebacterium glutamicum nationallicence l -phenylalanine nationallicence Shikimate nationallicence Glucose-PTS nationallicence Metabolic engineering nationallicence Zhang Chuanzhi Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Zhang Junli Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Kang Zhen Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Du Guocheng Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Chen Jian Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 787-797 1367-5435 42:5<787 2015 42 10295 https://doi.org/10.1007/s10295-015-1593-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1593-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Chuanzhi Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Zhang Junli Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Kang Zhen Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Du Guocheng Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Chen Jian Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/5(2015-05-01), 787-797 1367-5435 42:5<787 2015 42 10295