caa a22 4500 606213104 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1562-9 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1562-9 Interactive performances of betaine on the metabolic processes of Pseudomonas denitrificans [Elektronische Daten] [Wei Xia, Wei-fu Peng, Wei Chen, Kun-tai Li] The performances of betaine on the metabolic processes of vitamin B12-producing Pseudomonas denitrificans were investigated in this paper. The results showed that betaine was an indispensable methyl-group donor for vitamin B12 biosynthesis, but large amounts of the extracellular glycine accompanied by betaine metabolism would impose a severe restriction on the cell growth of P. denitrificans. By further using a comparative metabolomics approach coupled with intracellular free amino acids analysis for the fermentation processes with betaine addition (10g/l) or not, it was found that betaine could highly strengthen the formation of some key precursors and intermediates facilitating vitamin B12 biosynthesis, such as δ-aminolevulinic acid (ALA, the first precursor of vitamin B12), glutamate (an intermediate of ALA via C5 pathway), glycine (an intermediate of ALA via C4 pathway), and methionine (directly participating in the methylation reaction involved in vitamin B12 biosynthetic pathway). Therefore, the performances of betaine on P. denitrificans metabolic processes were not only serving as a decisive methyl-group donor for vitamin B12 biosynthesis, but also playing a powerfully promoting role in the generation of vitamin B12 precursors and intermediates. Society for Industrial Microbiology and Biotechnology, 2014 Pseudomonas denitrificans nationallicence Vitamin B12 nationallicence Betaine nationallicence Metabolic process nationallicence Xia Wei Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, 330045, Nanchang, China aut Peng Wei-fu Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, 330045, Nanchang, China aut Chen Wei Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, 330045, Nanchang, China aut Li Kun-tai Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, 330045, Nanchang, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 273-278 1367-5435 42:2<273 2015 42 10295 https://doi.org/10.1007/s10295-014-1562-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1562-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xia Wei Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, 330045, Nanchang, China aut NATIONALLICENCE 700 1- Peng Wei-fu Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, 330045, Nanchang, China aut NATIONALLICENCE 700 1- Chen Wei Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, 330045, Nanchang, China aut NATIONALLICENCE 700 1- Li Kun-tai Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, 330045, Nanchang, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 273-278 1367-5435 42:2<273 2015 42 10295