caa a22 4500 606213120 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1550-0 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1550-0 Bioconversion of α-pinene by a novel cold-adapted fungus Chrysosporium pannorum [Elektronische Daten] [Mariusz Trytek, Krzysztof Jędrzejewski, Jan Fiedurek] The psychrotrophic fungus Chrysosporium pannorum A-1 is reported for the first time as a novel biocatalyst for O2-promoted oxidation of α-pinene. GC-MS analysis indicated that the main products of the reaction were compounds of a high commercial value, verbenol (1) and verbenone (2). Exponentially growing cells (days 2-3) were about twice as active as cells in the late stationary phase in terms of the total concentration of products. The highest yields of 1 and 2 were obtained using three-day and two-day-old mycelia and a medium containing 1.5 and 1% (v/v) of the substrate, respectively. The optimal time for the bioconversion of α-pinene varied from 1 to 3days, and depended on the kind of product desired. Most of 1 was produced at a relatively high concentration of 360mg/L after the first six hours of α-pinene bioconversion [with an average yield of 69mg/(gdry cellL aqueous phase)]. The oxidative activity of C. pannorum was identified across a wide temperature range of 5-25°C, 10°C being the optimum for the production of 1 and 20°C for the production of 2. Sequential addition of the substrate during 3days of the biotransformation resulted in a significant increase in 1 and 2 up to 722 and 176mg/L, respectively, and a 2-fold enhancement of product yield as compared to bioconversion with a single supply of α-pinene. The concentration of total conversion products in the culture medium reached 1.33g/L [which corresponded product yield of 225mg/(g dry cell L)]. This represents probably the most promising result reported to date for oxidative biotransformation of α-pinene by a wild-type microorganism. The Author(s), 2014 Biotransformation nationallicence Chrysosporium nationallicence Pinene nationallicence Psychrotrophs nationallicence Terpenes nationallicence Verbenol nationallicence Verbenone nationallicence Trytek Mariusz Department of Industrial Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka St. 19, 20-033, Lublin, Poland aut Jędrzejewski Krzysztof Department of Industrial Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka St. 19, 20-033, Lublin, Poland aut Fiedurek Jan Department of Industrial Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka St. 19, 20-033, Lublin, Poland aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 181-188 1367-5435 42:2<181 2015 42 10295 https://doi.org/10.1007/s10295-014-1550-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1550-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Trytek Mariusz Department of Industrial Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka St. 19, 20-033, Lublin, Poland aut NATIONALLICENCE 700 1- Jędrzejewski Krzysztof Department of Industrial Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka St. 19, 20-033, Lublin, Poland aut NATIONALLICENCE 700 1- Fiedurek Jan Department of Industrial Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka St. 19, 20-033, Lublin, Poland aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 181-188 1367-5435 42:2<181 2015 42 10295