caa a22 4500 606213163 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1566-5 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1566-5 Polyethyleneimine-grafted collagen fiber as a carrier for cell immobilization [Elektronische Daten] [Deyi Zhu, Xia Li, Xuepin Liao, Bi Shi] Collagen fiber (CF), an abundant natural biopolymer, features many favorable properties that make it a potential carrier for cell immobilization. In the present investigation, CF was grafted with polyethyleneimine (PEI) using glutaraldehyde (GA) as the cross-linking agent, resulting in the formation of a novel CF based carrier (CF-PEI). The properties of CF-PEI as a carrier were evaluated by the immobilization of Microbacterium arborescens (CICC 20196), which has glucose isomerase (EC 5.3.1.5) activity. It was found that M. arborescens cells immobilized on CF-PEI exhibited higher glucose isomerization than those using activated carbon or anion exchange resin as the carriers. The Michaelis constant (K m) of the isomerization reaction for the CF-PEI-immobilized M. arborescens cells was 0.528mol/L, which was slightly higher than that of free cells (0.473mol/L). In addition, the apparent activation energies (E a) of free and immobilized cells on CF-PEI were almost the same at 60kJ/mol. In an isomerization reaction of glucose to fructose in a fixed-bed reactor, CF-PEI-immobilized M. arborescens cells showed appreciable activity and operational stability. The corresponding isomerization ratio was as high as 41% for 20days, and the half-life was about 40days. Society for Industrial Microbiology and Biotechnology, 2014 Collagen fiber nationallicence Polyethyleneimine nationallicence Cell immobilization nationallicence Microbacterium arborescens nationallicence Glucose isomerization nationallicence Zhu Deyi Department of Biomass Chemistry and Engineering, Sichuan University, No. 24 South Section1, Yihuan Road, 610065, Chengdu, People's Republic of China aut Li Xia Department of Biomass Chemistry and Engineering, Sichuan University, No. 24 South Section1, Yihuan Road, 610065, Chengdu, People's Republic of China aut Liao Xuepin Department of Biomass Chemistry and Engineering, Sichuan University, No. 24 South Section1, Yihuan Road, 610065, Chengdu, People's Republic of China aut Shi Bi Department of Biomass Chemistry and Engineering, Sichuan University, No. 24 South Section1, Yihuan Road, 610065, Chengdu, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 189-196 1367-5435 42:2<189 2015 42 10295 https://doi.org/10.1007/s10295-014-1566-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1566-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Deyi Department of Biomass Chemistry and Engineering, Sichuan University, No. 24 South Section1, Yihuan Road, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Li Xia Department of Biomass Chemistry and Engineering, Sichuan University, No. 24 South Section1, Yihuan Road, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Liao Xuepin Department of Biomass Chemistry and Engineering, Sichuan University, No. 24 South Section1, Yihuan Road, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Shi Bi Department of Biomass Chemistry and Engineering, Sichuan University, No. 24 South Section1, Yihuan Road, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 189-196 1367-5435 42:2<189 2015 42 10295