caa a22 4500 60621318X CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1558-5 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1558-5 Biocatalytic deracemisation of aliphatic β-hydroxy esters: Improving the enantioselectivity by optimisation of reaction parameters [Elektronische Daten] [Sowmyalakshmi Venkataraman, Anju Chadha] Optically pure aliphatic β-hydroxy esters were prepared from their racemates by deracemisation using the biocatalyst Candida parapsilosis ATCC 7330. High optical purity (up to >99%) and good yields (up to 71%) of the product secondary alcohols were obtained. This study highlights the importance of optimization of reaction conditions using ethyl-3-hydroxybutanoate as the model substrate to improve the enantioselectivity (enantiomeric excess from 9 to 98%). The present study emphasises the broad substrate scope of the biocatalyst towards deracemisation. This is the first report of Candida parapsilosis ATCC 7330-mediated deracemisation of various alkyl-3-hydroxybutanoates to produce either the (R)-enantiomers (methyl, ethyl, propyl, butyl, t-butyl, allyl-3-hydroxybutanoates) or (S)-enantiomers (pentyl, iso-amyl and iso-propyl-3-hydroxybutanoates). Society for Industrial Microbiology and Biotechnology, 2014 Deracemisation nationallicence Candida parapsilosis ATCC 7330 nationallicence Aliphatic β-hydroxy esters nationallicence Alkyl-3-hydroxybutanoates nationallicence Optimisation studies nationallicence Venkataraman Sowmyalakshmi Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, 600 036, Chennai, India aut Chadha Anju Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, 600 036, Chennai, India aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 173-180 1367-5435 42:2<173 2015 42 10295 https://doi.org/10.1007/s10295-014-1558-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1558-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Venkataraman Sowmyalakshmi Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, 600 036, Chennai, India aut NATIONALLICENCE 700 1- Chadha Anju Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, 600 036, Chennai, India aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 173-180 1367-5435 42:2<173 2015 42 10295