caa a22 4500 606213198 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1568-3 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1568-3 Lime application for the efficient production of nutraceutical glucooligosaccharides from Leuconostoc mesenteroides NRRL B-742 (ATCC13146) [Elektronische Daten] [Young Moon, Lee Madsen, Chang-Ho Chung, Doman Kim, Donal Day] We have previously demonstrated the production of glucooligosaccharides via a fermentation of sucrose with Leuconostoc mesenteroides NRRL B-742 using sodium hydroxide (NaOH) to control the pH. Because NaOH is expensive, we sought to minimize the cost of our process by substituting hydrated lime and saccharate of lime (lime sucrate) in its place. The yield of glucooligosaccharides using either 5% lime (41.4±0.5g/100g) or 5% lime sucrate (40.0±1.4g/100g) were both similar to the NaOH control (42.4±1.5g/100g). Based on this, it appears that the cost associated with pH control in our process can be reduced by a factor of approximately 2.4 using lime instead of NaOH. Because our chromatographic stage is based on a Ca2+-form resin to separate glucooligosaccharides, the use of lime not only negates the need for costly de-salting via ion-exchange (elimination of two ion-exchange sections) prior to separation, but also greatly reduces the resin regeneration cost. Society for Industrial Microbiology and Biotechnology, 2014 Leuconostoc mesenteroides NRRL B-742 nationallicence Glucooligosaccharides nationallicence Calcium hydroxide nationallicence Lime nationallicence Saccharate nationallicence Sucrate nationallicence Moon Young Audubon Sugar Institute, Louisiana State University Agricultural Center, 3,845 Highway 75, 70776, Saint Gabriel, LA, USA aut Madsen Lee Audubon Sugar Institute, Louisiana State University Agricultural Center, 3,845 Highway 75, 70776, Saint Gabriel, LA, USA aut Chung Chang-Ho Department of Culinary Science and Food Service Management, Sejong University, 98 Gunja-dong, Gwangjin-gu, 143-747, Seoul, South Korea aut Kim Doman Graduate School of International Agricultural Technology and Institutes of Green Bio Science and Technology, Seoul National University, 232-916, Pyeongchang-gun, Gangwon-do, South Korea aut Day Donal Audubon Sugar Institute, Louisiana State University Agricultural Center, 3,845 Highway 75, 70776, Saint Gabriel, LA, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 279-285 1367-5435 42:2<279 2015 42 10295 https://doi.org/10.1007/s10295-014-1568-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1568-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moon Young Audubon Sugar Institute, Louisiana State University Agricultural Center, 3,845 Highway 75, 70776, Saint Gabriel, LA, USA aut NATIONALLICENCE 700 1- Madsen Lee Audubon Sugar Institute, Louisiana State University Agricultural Center, 3,845 Highway 75, 70776, Saint Gabriel, LA, USA aut NATIONALLICENCE 700 1- Chung Chang-Ho Department of Culinary Science and Food Service Management, Sejong University, 98 Gunja-dong, Gwangjin-gu, 143-747, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Doman Graduate School of International Agricultural Technology and Institutes of Green Bio Science and Technology, Seoul National University, 232-916, Pyeongchang-gun, Gangwon-do, South Korea aut NATIONALLICENCE 700 1- Day Donal Audubon Sugar Institute, Louisiana State University Agricultural Center, 3,845 Highway 75, 70776, Saint Gabriel, LA, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 279-285 1367-5435 42:2<279 2015 42 10295