caa a22 4500 606213201 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1556-7 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1556-7 Genomic reconstruction to improve bioethanol and ergosterol production of industrial yeast Saccharomyces cerevisiae [Elektronische Daten] [Ke Zhang, Mengmeng Tong, Kehui Gao, Yanan Di, Pinmei Wang, Chunfang Zhang, Xuechang Wu, Daoqiong Zheng] Baker's yeast (Saccharomyces cerevisiae) is the common yeast used in the fields of bread making, brewing, and bioethanol production. Growth rate, stress tolerance, ethanol titer, and byproducts yields are some of the most important agronomic traits of S. cerevisiae for industrial applications. Here, we developed a novel method of constructing S. cerevisiae strains for co-producing bioethanol and ergosterol. The genome of an industrial S. cerevisiae strain, ZTW1, was first reconstructed through treatment with an antimitotic drug followed by sporulation and hybridization. A total of 140 mutants were selected for ethanol fermentation testing, and a significant positive correlation between ergosterol content and ethanol production was observed. The highest performing mutant, ZG27, produced 7.9% more ethanol and 43.2% more ergosterol than ZTW1 at the end of fermentation. Chromosomal karyotyping and proteome analysis of ZG27 and ZTW1 suggested that this breeding strategy caused large-scale genome structural variations and global gene expression diversities in the mutants. Genetic manipulation further demonstrated that the altered expression activity of some genes (such as ERG1, ERG9, and ERG11) involved in ergosterol synthesis partly explained the trait improvement in ZG27. Society for Industrial Microbiology and Biotechnology, 2014 Saccharomyces cerevisiae nationallicence Bioethanol nationallicence Ergosterol nationallicence Genome reconstruction nationallicence Tolerance nationallicence Zhang Ke Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Tong Mengmeng Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Gao Kehui Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Di Yanan Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Wang Pinmei Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Zhang Chunfang Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Wu Xuechang Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Zheng Daoqiong Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 207-218 1367-5435 42:2<207 2015 42 10295 https://doi.org/10.1007/s10295-014-1556-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1556-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Ke Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Tong Mengmeng Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Gao Kehui Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Di Yanan Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Wang Pinmei Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Chunfang Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Wu Xuechang Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Daoqiong Zhejiang University, Zijingang Campus, 866 Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 207-218 1367-5435 42:2<207 2015 42 10295