caa a22 4500 60621321X CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1555-8 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1555-8 Constructing a recombinant hyaluronic acid biosynthesis operon and producing food-grade hyaluronic acid in Lactococcus lactis [Elektronische Daten] [Juzheng Sheng, Peixue Ling, Fengshan Wang] Hyaluronic acid (HA), a natural high molecular weight polysaccharide, is produced by Streptococcus zooepidemicus. However, Streptococcus has several drawbacks including its potential to produce exotoxins, so there is demand for an alternative HA source. Here, a recombinant HA biosynthesis operon, as well as the HA biosynthesis operon of S. zooepidemicus were introduced into L. lactis using the nisin-controlled expression system, respectively. HA was successfully synthesized by recombinant L. lactis. Furthermore, overexpression of the endogenous enzymes directing the synthesis of precursor sugars was effective at increasing HA production, and increasing the supply of UDP-activated monosaccharide donors aided synthesis of monodisperse HA polysaccharides. Besides GRAS host strain (L. lactis) and NICE system, the selecting marker (lacF gene) of the recombinant strain is also food grade. Therefore, HA produced by recombinant L. lactis overcomes the problems associated with Streptococcus and provides a source of food-grading HA appropriate for widespread biotechnological applications. Society for Industrial Microbiology and Biotechnology, 2014 Food grade nationallicence Hyaluronic acid nationallicence Hyaluronan synthase nationallicence UDP-glucose-6-dehydrogenase nationallicence Streptococcus zooepidemicus nationallicence Lactococcus lactis nationallicence The nisin-controlled gene expression (NICE) system nationallicence Sheng Juzheng Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, 250012, Jinan, China aut Ling Peixue Shandong Academy of Pharmaceutical Science, 250101, Jinan, China aut Wang Fengshan Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, 250012, Jinan, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 197-206 1367-5435 42:2<197 2015 42 10295 https://doi.org/10.1007/s10295-014-1555-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1555-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sheng Juzheng Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, 250012, Jinan, China aut NATIONALLICENCE 700 1- Ling Peixue Shandong Academy of Pharmaceutical Science, 250101, Jinan, China aut NATIONALLICENCE 700 1- Wang Fengshan Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Sciences, Shandong University, 250012, Jinan, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 197-206 1367-5435 42:2<197 2015 42 10295