caa a22 4500 606213228 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1557-6 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1557-6 Antibacterial activity of a cell wall hydrolase from Lactobacillus paracasei NRRL B-50314 produced by recombinant Bacillus megaterium [Elektronische Daten] [Siqing Liu, Joseph Rich, Amber Anderson] The cell-free supernatant (CFS) from Lactobacillus paracasei NRRL B-50314 culture has been previously reported as containing antibacterial activity against a wide variety of Gram-positive bacteria. The CFS protein gel slice corresponding to antibacterial activities was subjected to trypsin digestion and ion trap MASS (Gel/LC-MS/MS) analysis. BlastP search of the resulted IQAVISIAEQQIGKP sequence led to a hypothetical cell-wall associated hydrolase (designated as CWH here) from Lactobacillus paracasei ATCC 25302. Further analyses of CWH revealed that the IQAVISIAEQQIGKP belongs to a highly conserved region of the NlpC/P60 superfamily. The L. paracasei NRRL B-50314 CWH gene, cloned in pStrepHIS1525CWH477, was introduced into Bacillus megaterium MS 941. The production of CWH477 protein was induced by xylose. The CWH477 protein was purified by using NiNTA column, and elution fraction E2 showed highest antibacterial activity. This study and bioinformatics analyses suggested that the antibacterial activity of CWH could originate from its cell wall degrading enzymatic function. Springer-Verlag (outside the USA), 2014 Lactobacillus paracasei nationallicence Antibacterial protein nationallicence Cell-wall associated hydrolase nationallicence Bacillus megaterium nationallicence Liu Siqing RPT Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815N. University St, 61604, Peoria, IL, USA aut Rich Joseph RPT Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815N. University St, 61604, Peoria, IL, USA aut Anderson Amber RPT Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815N. University St, 61604, Peoria, IL, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 229-235 1367-5435 42:2<229 2015 42 10295 https://doi.org/10.1007/s10295-014-1557-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1557-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Siqing RPT Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815N. University St, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Rich Joseph RPT Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815N. University St, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Anderson Amber RPT Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815N. University St, 61604, Peoria, IL, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 229-235 1367-5435 42:2<229 2015 42 10295