caa a22 4500 606213236 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10295-014-1563-8 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1563-8 Deletion of genes involved in glutamate metabolism to improve poly-gamma-glutamic acid production in B. amyloliquefaciens LL3 [Elektronische Daten] [Wei Zhang, Yulian He, Weixia Gao, Jun Feng, Mingfeng Cao, Chao Yang, Cunjiang Song, Shufang Wang] Here, we attempted to elevate poly-gamma-glutamic acid (γ-PGA) production by modifying genes involved in glutamate metabolism in Bacillus amyloliquefaciens LL3. Products of rocR, rocG and gudB facilitate the conversion from glutamate to 2-oxoglutarate in Bacillus subtillis. The gene odhA is responsible for the synthesis of a component of the 2-oxoglutarate dehydrogenase complex that catalyzes the oxidative decarboxylation of 2-oxoglutarate to succinyl coenzyme A. In-frame deletions of these four genes were performed. In shake flask experiments the gudB/rocG double mutant presented enhanced production of γ-PGA, a 38% increase compared with wild type. When fermented in a 5-L fermenter with pH control, the γ-PGA yield of the rocR mutant was increased to 5.83g/L from 4.55g/L for shake flask experiments. The gudB/rocG double mutant produced 5.68g/L γ-PGA compared with that of 4.03g/L for the wild type, a 40% increase. Those results indicated the possibility of improving γ-PGA production by modifying glutamate metabolism, and identified potential genetic targets to improve γ-PGA production. Society for Industrial Microbiology and Biotechnology, 2014 Gamma-PGA nationallicence Glutamate metabolism nationallicence B. amyloliquefaciens nationallicence Markerless gene replacement nationallicence Zhang Wei Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut He Yulian Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut Gao Weixia Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut Feng Jun Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut Cao Mingfeng Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, USA aut Yang Chao Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut Song Cunjiang Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut Wang Shufang State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 297-305 1367-5435 42:2<297 2015 42 10295 https://doi.org/10.1007/s10295-014-1563-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1563-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Wei Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- He Yulian Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Gao Weixia Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Feng Jun Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Cao Mingfeng Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, USA aut NATIONALLICENCE 700 1- Yang Chao Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Song Cunjiang Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Wang Shufang State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/2(2015-02-01), 297-305 1367-5435 42:2<297 2015 42 10295