caa a22 4500 606213279 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10295-015-1674-x doi (NATIONALLICENCE)springer-10.1007/s10295-015-1674-x Fatty alcohols production by oleaginous yeast [Elektronische Daten] [Sandy Fillet, Jordi Gibert, Beatriz Suárez, Armando Lara, Carmen Ronchel, José Adrio] We have engineered Rhodosporidium toruloides to produce fatty alcohols by expressing a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8. Production of fatty alcohols in flasks was achieved in different fermentation media at titers ranging from 0.2 to 2g/L. In many of the conditions tested, more than 80% of fatty alcohols were secreted into the cultivation broth. Through fed-batch fermentation in 7L bioreactors, over 8g/L of C16-C18 fatty alcohols were produced using sucrose as the substrate. This is the highest titer ever reported on microbial production of fatty alcohols to date. The Author(s), 2015 Fatty alcohols nationallicence Metabolic engineering nationallicence Fatty acyl-CoA reductase nationallicence Rhodosporidium toruloides nationallicence Fillet Sandy Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut Gibert Jordi Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut Suárez Beatriz Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut Lara Armando Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut Ronchel Carmen Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut Adrio José Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1463-1472 1367-5435 42:11<1463 2015 42 10295 https://doi.org/10.1007/s10295-015-1674-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1674-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fillet Sandy Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut NATIONALLICENCE 700 1- Gibert Jordi Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut NATIONALLICENCE 700 1- Suárez Beatriz Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut NATIONALLICENCE 700 1- Lara Armando Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut NATIONALLICENCE 700 1- Ronchel Carmen Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut NATIONALLICENCE 700 1- Adrio José Neol Biosolutions SA, Avda. Innovación, 1, Edificio BIC-213, Parque Tecnológico de La Salud, 18016, Granada, Spain aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1463-1472 1367-5435 42:11<1463 2015 42 10295