caa a22 4500 606213309 CHVBK 20210128101046.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10295-015-1676-8 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1676-8 Expression and display of a novel thermostable esterase from Clostridium thermocellum on the surface of Bacillus subtilis using the CotB anchor protein [Elektronische Daten] [Huayou Chen, Tianxi Zhang, Jinru Jia, Ake Vastermark, Rui Tian, Zhong Ni, Zhi Chen, Keping Chen, Shengli Yang] Esterases expressed in microbial hosts are commercially valuable, but their applications are limited due to high costs of production and harsh industrial processes involved. In this study, the esterase-DSM (from Clostridium thermocellum) was expressed and successfully displayed on the spore surface, and the spore-associated esterase was confirmed by western blot analysis and activity measurements. The optimal temperature and pH of spore surface-displayed DSM was 60 and 8.5°C, respectively. It also demonstrates a broad temperature and pH optimum in the range of 50-70, 7-9.5°C. The spore surface-displayed esterase-DSM retained 78, 68% of its original activity after 5h incubation at 60 and 70°C, respectively, which was twofold greater activity than that of the purified DSM. The recombinant spores has high activity and stability in DMSO, which was 49% higher than the retained activity of the purified DSM in DMSO (20% v/v), and retained 65.2% of activity after 7h of incubation in DMSO (20% v/v). However, the recombinant spores could retain 77% activity after 3 rounds of recycling. These results suggest that enzyme displayed on the surface of the Bacillus subtilis spore could serve as an effective approach for enzyme immobilization. Society for Industrial Microbiology and Biotechnology, 2015 Thermophilic nationallicence Esterase nationallicence Bacillus subtilis nationallicence CotB nationallicence Surface display nationallicence Chen Huayou Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut Zhang Tianxi Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut Jia Jinru Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut Vastermark Ake Division of Biological Sciences, University of California at San Diego, 92093-0116, La Jolla, CA, USA aut Tian Rui Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut Ni Zhong Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut Chen Zhi Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut Chen Keping Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut Yang Shengli Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1439-1448 1367-5435 42:11<1439 2015 42 10295 https://doi.org/10.1007/s10295-015-1676-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1676-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Huayou Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut NATIONALLICENCE 700 1- Zhang Tianxi Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut NATIONALLICENCE 700 1- Jia Jinru Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut NATIONALLICENCE 700 1- Vastermark Ake Division of Biological Sciences, University of California at San Diego, 92093-0116, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Tian Rui Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut NATIONALLICENCE 700 1- Ni Zhong Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut NATIONALLICENCE 700 1- Chen Zhi Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut NATIONALLICENCE 700 1- Chen Keping Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut NATIONALLICENCE 700 1- Yang Shengli Institute of Life Sciences, Jiangsu University, 212013, Zhenjiang, Jiangsu Province, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1439-1448 1367-5435 42:11<1439 2015 42 10295