caa a22 4500 606213333 CHVBK 20210128101047.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10295-015-1678-6 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1678-6 Development of engineered Escherichia coli whole-cell biocatalysts for high-level conversion of l -lysine into cadaverine [Elektronische Daten] [Young Oh, Kyoung-Hee Kang, Mi Kwon, Jae Choi, Jeong Joo, Seung Lee, Yung-Hun Yang, Bong Song, Il-Kwon Kim, Ki-Hoon Yoon, Kyungmoon Park, Si Park] A whole-cell biocatalytic system for the production of cadaverine from l-lysine has been developed. Among the investigated lysine decarboxylases from different microorganisms, Escherichia coli LdcC showed the best performance on cadaverine synthesis when E. coli XL1-Blue was used as the host strain. Six different strains of E. coli expressing E. coli LdcC were investigated and recombinant E. coli XL1-Blue, BL21(DE3) and W were chosen for further investigation since they showed higher conversion yield of lysine into cadaverine. The effects of substrate pH, substrate concentrations, buffering conditions, and biocatalyst concentrations have been investigated. Finally, recombinant E. coli XL1-Blue concentrated to an OD600 of 50, converted 192.6g/L (1317mM) of crude lysine solution, obtained from an actual lysine manufacturing process, to 133.7g/L (1308mM) of cadaverine with a molar yield of 99.90%. The whole-cell biocatalytic system described herein is expected to be applicable to the development of industrial bionylon production process. Society for Industrial Microbiology and Biotechnology, 2015 Cadaverine nationallicence l -Lysine nationallicence Whole-cell conversion nationallicence Bio-catalyst nationallicence Recombinant E. coli nationallicence Oh Young Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut Kang Kyoung-Hee Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut Kwon Mi Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, 449-728, Yongin, Gyeonggido, Republic of Korea aut Choi Jae Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut Joo Jeong Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut Lee Seung Department of Biotechnology and Bioengineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, 500-757, Gwangju, Republic of Korea aut Yang Yung-Hun Department of Microbial Engineering, College of Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Song Bong Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut Kim Il-Kwon Bio R&D Center, Paik Kwang Industry Co., 57 Oehang-4 Gil, Gunsan-si, Jeollabukdo, Republic of Korea aut Yoon Ki-Hoon Bio R&D Center, Paik Kwang Industry Co., 57 Oehang-4 Gil, Gunsan-si, Jeollabukdo, Republic of Korea aut Park Kyungmoon Department of Biological and Chemical Engineering, Hongik University, 339-701, Chungnam, Republic of Korea aut Park Si Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, 449-728, Yongin, Gyeonggido, Republic of Korea aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1481-1491 1367-5435 42:11<1481 2015 42 10295 https://doi.org/10.1007/s10295-015-1678-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1678-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oh Young Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Kang Kyoung-Hee Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Kwon Mi Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, 449-728, Yongin, Gyeonggido, Republic of Korea aut NATIONALLICENCE 700 1- Choi Jae Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Joo Jeong Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Lee Seung Department of Biotechnology and Bioengineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, 500-757, Gwangju, Republic of Korea aut NATIONALLICENCE 700 1- Yang Yung-Hun Department of Microbial Engineering, College of Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Song Bong Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, P.O. Box 107, 305-600, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Il-Kwon Bio R&D Center, Paik Kwang Industry Co., 57 Oehang-4 Gil, Gunsan-si, Jeollabukdo, Republic of Korea aut NATIONALLICENCE 700 1- Yoon Ki-Hoon Bio R&D Center, Paik Kwang Industry Co., 57 Oehang-4 Gil, Gunsan-si, Jeollabukdo, Republic of Korea aut NATIONALLICENCE 700 1- Park Kyungmoon Department of Biological and Chemical Engineering, Hongik University, 339-701, Chungnam, Republic of Korea aut NATIONALLICENCE 700 1- Park Si Department of Environmental Engineering and Energy, Myongji University, 116 Myongji-ro, Cheoin-gu, 449-728, Yongin, Gyeonggido, Republic of Korea aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1481-1491 1367-5435 42:11<1481 2015 42 10295