caa a22 4500 606213341 CHVBK 20210128101047.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10295-015-1688-4 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1688-4 Effects of heterologous expression of phosphoenolpyruvate carboxykinase and phosphoenolpyruvate carboxylase on organic acid production in Aspergillus carbonarius [Elektronische Daten] [Lei Yang, Mette Lübeck, Peter Lübeck] Aspergillus carbonarius has a potential as a cell factory for production of various organic acids. In this study, the organic acid profile of A. carbonarius was investigated under different cultivation conditions. Moreover, two heterologous genes, pepck and ppc, which encode phosphoenolpyruvate carboxykinase in Actinobacillus succinogenes and phosphoenolpyruvate carboxylase in Escherichia coli, were inserted individually and in combination in A. carbonarius to enhance the carbon flux toward the reductive TCA branch. Results of transcription analysis and measurement of enzyme activities of phosphoenolpyruvate carboxykinase and phosphoenolpyruvate carboxylase in the corresponding single and double transformants demonstrated that the two heterologous genes were successfully expressed in A. carbonarius. The production of citric acid increased in all the transformants in both glucose- and xylose-based media at pH higher than 3 but did not increase in the pH non-buffered cultivation compared with the wild type. The Author(s), 2015 Aspergillus carbonarius nationallicence Phosphoenolpyruvate carboxykinase nationallicence Phosphoenolpyruvate carboxylase nationallicence Citric acid nationallicence Organic acids nationallicence Yang Lei Section for Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University Copenhagen, A. C. Meyers Vaenge 15, 2450, Copenhagen SV, Denmark aut Lübeck Mette Section for Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University Copenhagen, A. C. Meyers Vaenge 15, 2450, Copenhagen SV, Denmark aut Lübeck Peter Section for Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University Copenhagen, A. C. Meyers Vaenge 15, 2450, Copenhagen SV, Denmark aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1533-1545 1367-5435 42:11<1533 2015 42 10295 https://doi.org/10.1007/s10295-015-1688-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1688-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Lei Section for Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University Copenhagen, A. C. Meyers Vaenge 15, 2450, Copenhagen SV, Denmark aut NATIONALLICENCE 700 1- Lübeck Mette Section for Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University Copenhagen, A. C. Meyers Vaenge 15, 2450, Copenhagen SV, Denmark aut NATIONALLICENCE 700 1- Lübeck Peter Section for Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University Copenhagen, A. C. Meyers Vaenge 15, 2450, Copenhagen SV, Denmark aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1533-1545 1367-5435 42:11<1533 2015 42 10295