caa a22 4500 606213368 CHVBK 20210128101047.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10295-015-1653-2 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1653-2 Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library [Elektronische Daten] [Yongfei Hu, Yinghui Liu, Jing Li, Yanbin Feng, Na Lu, Baoli Zhu, Song Xue] A low-temperature-active alkaline esterase, Est12, from a marine sediment metagenomic fosmid library was identified. Est12 prefers short- and middle-chain p-nitrophenol esters as substrate with optimum temperature and pH value of 50°C and 9.0, respectively, and nearly 50% of maximum activity retained at 5°C. The hydrolysis activity of Est12 was stable at 40°C. Ca2+ especially activated the activity of Est12 to about 151% of the control. DEPC and PMSF inhibited the activity of Est12 to 34 and 25%, respectively. In addition, Est12 was more tolerable to methanol compared to other organic solvents tested. The crystal structure of Est12 at 1.39 Å resolution showed that the cap domain which is composed of an α-helix and a flexible region resulted in a relatively wide spectrum of substrate, with p-nitrophenol caproate as the preferred one. Furthermore, the flexible cap domain and the high percentage of Gly, Ser, and Met may play important roles in the adaptation of Est12 to low temperature. Society for Industrial Microbiology and Biotechnology, 2015 Marine sediment nationallicence Fosmid library nationallicence Esterase nationallicence Low-temperature active nationallicence Crystal structure nationallicence Hu Yongfei CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut Liu Yinghui Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China aut Li Jing CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut Feng Yanbin Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China aut Lu Na CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut Zhu Baoli CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut Xue Song Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1449-1461 1367-5435 42:11<1449 2015 42 10295 https://doi.org/10.1007/s10295-015-1653-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1653-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Yongfei CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Liu Yinghui Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 700 1- Li Jing CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Feng Yanbin Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 700 1- Lu Na CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Zhu Baoli CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Xue Song Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/11(2015-11-01), 1449-1461 1367-5435 42:11<1449 2015 42 10295