caa a22 4500 60621559X CHVBK 20210128101059.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00701-014-2339-7 doi (NATIONALLICENCE)springer-10.1007/s00701-014-2339-7 Changes of fractional anisotropy (FA) and apparent diffusion coefficient (ADC) in the model of experimental acute hydrocephalus in rabbits [Elektronische Daten] [Maxim Shevtsov, Konstantin Senkevich, Alexander Kim, Kseniia Gerasimova, Tatyana Trofimova, Galina Kataeva, Sviatoslav Medvedev, Olga Smirnova, Zhanna Savintseva, Marina Martynova, Olga Bystrova, Emil Pitkin, Galina Yukina, William Khachatryan] Background: To study the integrity of white matter, we investigated the correlation between the changes in neuroradiological and morphological parameters in an animal model of acute obstructive hydrocephalus. Methods: Hydrocephalus was induced in New Zealand rabbits (n = 10) by stereotactic injection of kaolin into the lateral ventricles. Control animals received saline in place of kaolin (n = 10). The progression of hydrocephalus was assessed using magnetic resonance imaging. Regional fractional anisotropy (FA) and the apparent diffusion coefficient (ADC) were measured in several white matter regions before and after the infusion of kaolin. Morphology of myelinated nerve fibers as well as of the blood-brain barrier were studied with the help of transmission electron microscopy (TEM) and light microscopy. Results: Compared with control animals, kaolin injection into the ventricles resulted in a dramatic increase in ventricular volume with compression of basal cisterns, brain shift and periventricular edema (as observed on magnetic resonance imaging [MRI]). The values of ADC in the periventricular and periaqueductal areas significantly increased in the experimental group (P < 0.05). FA decreased by a factor of 2 in the zones of periventricular, periaqueductal white matter and corpus collosum. Histological analysis demonstrated the impairment of the white matter and necrobiotic changes in the cortex. Microsctructural alterations of the myelin fibers were further proved with the help of TEM. Blood-brain barrier ultrastructure assessment showed the loss of its integrity. Conclusions: The study demonstrated the correlation of the neuroradiological parameters with morphological changes. The abnormality of the FA and ADC parameters in the obstructive hydrocephalus represents a significant implication for the diagnostics and management of hydrocephalus in patients. Springer-Verlag Wien, 2015 Hydrocephalus nationallicence Magnetic resonance imaging nationallicence Fractional anisotropy nationallicence Apparent diffusion coefficient nationallicence White matter nationallicence Ventriculomegaly nationallicence Shevtsov Maxim A.L. Polenov Russian Scientific Research Institute of Neurosurgery, Mayakovsky str. 12, 191014, St. Petersburg, Russia aut Senkevich Konstantin I.P. Pavlov State Medical University, L. Tolstoi str. 6-8, 197022, St. Petersburg, Russia aut Kim Alexander A.L. Polenov Russian Scientific Research Institute of Neurosurgery, Mayakovsky str. 12, 191014, St. Petersburg, Russia aut Gerasimova Kseniia A.L. Polenov Russian Scientific Research Institute of Neurosurgery, Mayakovsky str. 12, 191014, St. Petersburg, Russia aut Trofimova Tatyana N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut Kataeva Galina N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut Medvedev Sviatoslav N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut Smirnova Olga N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut Savintseva Zhanna N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut Martynova Marina Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave. 4, 194064, St. Petersburg, Russia aut Bystrova Olga Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave. 4, 194064, St. Petersburg, Russia aut Pitkin Emil The Wharton School, University of Pennsylvania, 3730 Walnut St., 19104, Philadelphia, PA, USA aut Yukina Galina I.P. Pavlov State Medical University, L. Tolstoi str. 6-8, 197022, St. Petersburg, Russia aut Khachatryan William A.L. Polenov Russian Scientific Research Institute of Neurosurgery, Mayakovsky str. 12, 191014, St. Petersburg, Russia aut Acta Neurochirurgica Springer Vienna 157/4(2015-04-01), 689-698 0001-6268 157:4<689 2015 157 701 https://doi.org/10.1007/s00701-014-2339-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00701-014-2339-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shevtsov Maxim A.L. Polenov Russian Scientific Research Institute of Neurosurgery, Mayakovsky str. 12, 191014, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Senkevich Konstantin I.P. Pavlov State Medical University, L. Tolstoi str. 6-8, 197022, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Kim Alexander A.L. Polenov Russian Scientific Research Institute of Neurosurgery, Mayakovsky str. 12, 191014, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Gerasimova Kseniia A.L. Polenov Russian Scientific Research Institute of Neurosurgery, Mayakovsky str. 12, 191014, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Trofimova Tatyana N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Kataeva Galina N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Medvedev Sviatoslav N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Smirnova Olga N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Savintseva Zhanna N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences (RAS), Akademika Pavlova str. 9, 197376, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Martynova Marina Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave. 4, 194064, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Bystrova Olga Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky Ave. 4, 194064, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Pitkin Emil The Wharton School, University of Pennsylvania, 3730 Walnut St., 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Yukina Galina I.P. Pavlov State Medical University, L. Tolstoi str. 6-8, 197022, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Khachatryan William A.L. Polenov Russian Scientific Research Institute of Neurosurgery, Mayakovsky str. 12, 191014, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Acta Neurochirurgica Springer Vienna 157/4(2015-04-01), 689-698 0001-6268 157:4<689 2015 157 701