caa a22 4500 606217479 CHVBK 20210128101108.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11104-015-2503-8 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2503-8 Response of soil, leaf endosphere and phyllosphere bacterial communities to elevated CO2 and soil temperature in a rice paddy [Elektronische Daten] [Gaidi Ren, Chunwu Zhu, M. Alam, Takeshi Tokida, Hidemitsu Sakai, Hirofumi Nakamura, Yasuhiro Usui, Jianguo Zhu, Toshihiro Hasegawa, Zhongjun Jia] Aims: The objective of this study was to elucidate the composition of bacterial communities from the soil, and endosphere and phyllosphere of upper and lower leaves and clarify the responses to elevated CO2 and/or soil temperature. Methods: Using 454 pyrosequencing, the 16S rRNA gene was analyzed from various bacterial communities in a rice paddy that was exposed to different atmospheric CO2 concentrations (ambient, +200μmol.mol−1) and soil temperatures (ambient, +2°C). Results: The treatments of elevated temperature and elevated CO2 plus temperature exerted significant influence on the structure of bacterial communities from the lower leaf endosphere. A significant influence of elevated CO2 plus temperature on the community structure was also observed in the upper leaf phyllosphere. The richness and diversity of bacterial communities from the lower leaf phyllosphere, upper leaf endosphere, and upper leaf phyllosphere were significantly affected by elevated CO2 plus temperature. However, we did not observe any significant influence of all climate change treatments (elevated CO2, elevated temperature, and their combination) on the richness, diversity, and structure of soil bacterial communities. We also did not observe any significant effect of the single factor, elevated CO2, on the structure of the leaf endosphere and phyllosphere bacterial communities. Enterobacteriaceae and Xanthomonadaceae were the most shifted phylotypes in response to elevated temperature and elevated CO2 plus temperature. Conclusions: Soil bacterial communities were more resistant to the tested climate change factors compare with foliar bacterial communities. Temperature was a more important factor in shaping the structure of foliar bacterial communities compared with CO2. The response of leaf-associated bacterial communities could be influenced by the leaf location (upper leaf or lower leaf) within the rice plants and by the habitats (leaf endosphere or phyllosphere). Springer International Publishing Switzerland, 2015 Climate change nationallicence Global warming nationallicence Microbial community nationallicence Free air CO2 enrichment (FACE) nationallicence Pyrosequence nationallicence Leaf-associated bacteria nationallicence Ren Gaidi State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut Zhu Chunwu State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut Alam M. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut Tokida Takeshi Carbon andNutrientCycles Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Sakai Hidemitsu Agro-Meteorology Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Nakamura Hirofumi Taiyo Keiki Co. Ltd., 1-12-3, Nakajujo, 114-0032, Kita-ku, Tokyo, Japan aut Usui Yasuhiro Agro-Meteorology Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Zhu Jianguo State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut Hasegawa Toshihiro Agro-Meteorology Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut Jia Zhongjun State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 27-44 0032-079X 392:1-2<27 2015 392 11104 https://doi.org/10.1007/s11104-015-2503-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2503-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ren Gaidi State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Chunwu State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Alam M. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Tokida Takeshi Carbon andNutrientCycles Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Sakai Hidemitsu Agro-Meteorology Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Nakamura Hirofumi Taiyo Keiki Co. Ltd., 1-12-3, Nakajujo, 114-0032, Kita-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Usui Yasuhiro Agro-Meteorology Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Zhu Jianguo State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 700 1- Hasegawa Toshihiro Agro-Meteorology Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, 305-8604, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Jia Zhongjun State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, Jiangsu Province, People's Republic of China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 27-44 0032-079X 392:1-2<27 2015 392 11104