caa a22 4500 606217495 CHVBK 20210128101108.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11104-015-2443-3 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2443-3 Cadmium impairs iron homeostasis in Arabidopsis thaliana by increasing the polysaccharide contents and the iron-binding capacity of root cell walls [Elektronische Daten] [Shan Xu, Si Lin, Zhong Lai] Background and aims: Iron (Fe) deficiency is a well-known symptom of cadmium (Cd) toxicity. Here, the mechanisms underlying Cd impairment of Fe homeostasis in Arabidopsis were investigated. Methods: Arabidopsis plants were subjected to 0 (CK) or 10μM Cd treatments. After the treatments period, Fe concentrations, expression levels of Fe uptake- and translocation-related genes, polysaccharide concentrations, pectin methylesterase activities (PME) and the degree of pectin methylation (DM) were analyzed. Results: Fe concentrations in Cd-treated plants were lower in shoots, but higher in roots, especially in the root apoplasts, compared with the CK, and their leaves developed Fe-deficiency-like symptoms. However, Cd stimulated the root's Fe uptake activity and the expression of genes involved in Fe acquisition. In both treatments, the citrate concentrations and expression levels of Fe translocation-related gene were comparable. The Cd treatment significantly increased pectin and hemicelluloses concentrations in cell walls, while significantly decreased the DM by increasing the PME activity, which led to a higher binding capacity of the cell wall to Fe. Conclusions: The Cd-induced Fe accumulation in roots is mediated by increasing polysaccharide concentrations and decreasing the DM, which increases the Fe retention in roots and hampers its translocation to shoots. Springer International Publishing Switzerland, 2015 Cadmium stress nationallicence Iron deficiency nationallicence Cell wall nationallicence Pectin methylesterase nationallicence Polysaccharide nationallicence Cd : Cadmium nationallicence Fe : Iron nationallicence PME : Pectin methylesterase nationallicence DM : Degree of methyl-esterification nationallicence FCR : Ferric chelate reductase nationallicence IRT1 : Iron-regulated transporter 1 nationallicence FRO2 : Ferric reductase oxidase 2 nationallicence FRD3 : Ferric reductase defective 3 nationallicence Xu Shan State Forestry Administration Engineering Research Center of Chinese Fir, 350002, Fuzhou, China aut Lin Si State Forestry Administration Engineering Research Center of Chinese Fir, 350002, Fuzhou, China aut Lai Zhong Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 71-85 0032-079X 392:1-2<71 2015 392 11104 https://doi.org/10.1007/s11104-015-2443-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2443-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Shan State Forestry Administration Engineering Research Center of Chinese Fir, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Lin Si State Forestry Administration Engineering Research Center of Chinese Fir, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Lai Zhong Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 71-85 0032-079X 392:1-2<71 2015 392 11104