caa a22 4500 606217517 CHVBK 20210128101108.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11104-015-2464-y doi (NATIONALLICENCE)springer-10.1007/s11104-015-2464-y Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities [Elektronische Daten] [Yue Zhang, Xiu-Juan Wang, Shu-Yun Chen, Li-Yuan Guo, Ming-Lei Song, He Feng, Chen Li, Ji-Gang Bai] Aims: Ferulic acid (FA) accumulates in soil and inhibits plant growth. We examined whether the FA-degrading bacterium Bacillus methylotrophicus degrades FA in soil. Methods: B. methylotrophicus strain CSY-F1 was isolated and applied to unplanted soil and soil planted with Cucumis sativus (cucumber). We analyzed the effects of B. methylotrophicus on rhizosphere enzyme activities and antioxidant enzyme activities in cucumber and CSY-F1 exposed to FA. Results: CSY-F1 degraded FA in culture medium and in soil, giving rise to 4-vinyl guaiacol, vanillin, vanillic acid, and protocatechuic acid. When cucumber seedlings were grown in soil treated with FA, the activities of some soil enzymes decreased, and the malonaldehyde content in cucumber leaves increased. The addition of CSY-F1 to FA-treated soil increased the activities of these soil enzymes, decreased the FA concentration in the soil, and elevated the activities of some antioxidant enzymes in seedlings. Moreover, the levels of superoxide radical, hydrogen peroxide, and malonaldehyde were reduced in cucumber leaves. FA treatment increased the activities of antioxidant enzymes, including superoxide dismutase, catalase, and monodehydroascorbate, in CSY-F1. Conclusion: Bacillus methylotrophicus CSY-F1 has potential applications as an FA-degrading agent in soil, as it mitigates FA stress in cucumber seedlings by activating several antioxidant and soil enzymes. Springer International Publishing Switzerland, 2015 Antioxidant enzyme nationallicence Bacillus methylotrophicus nationallicence Ferulic acid nationallicence Soil enzyme nationallicence Zhang Yue State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Wang Xiu-Juan State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Chen Shu-Yun State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Guo Li-Yuan State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Song Ming-Lei State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Feng He State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Li Chen State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Bai Ji-Gang State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 309-321 0032-079X 392:1-2<309 2015 392 11104 https://doi.org/10.1007/s11104-015-2464-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2464-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yue State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xiu-Juan State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Chen Shu-Yun State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Guo Li-Yuan State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Song Ming-Lei State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Feng He State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Li Chen State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Bai Ji-Gang State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 309-321 0032-079X 392:1-2<309 2015 392 11104