caa a22 4500 606217606 CHVBK 20210128101108.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11104-015-2453-1 doi (NATIONALLICENCE)springer-10.1007/s11104-015-2453-1 Soil nitrogen pools and plant uptake at sub-zero soil temperature in a cool temperate forest soil: a field experiment using 15N labeling [Elektronische Daten] [Miki Ueda, Naoko Tokuchi, Tsutom Hiura] Aims: Winter biogeochemical processes have received considerable attention. Biological processes (e.g., microbial respiration and plant photosynthesis) do not cease, even at sub-zero temperatures. However, our knowledge of plant nitrogen (N) uptake at sub-zero soil temperatures is particularly limited for deciduous plant species, which do not have leaves during winter. We investigated plant N uptake by evergreen and deciduous species and soil N processes during sub-zero soil temperatures in cool temperate forest soil. Methods: Isotopically labelled nitrate (NO3-15N) was injected into soil as a tracer of plant uptake and soil N dynamics at sub-zero temperature soil at a cool temperate field site. Results: Over a period of 41days, 6-48mg/kg DW−1 of 15N accumulated in evergreen species and deciduous tree species. Furthermore, the 15N content in ammonium increased, suggesting ammonium production at sub-zero soil temperatures. The increase in 15NH4 was positively correlated with soil moisture, indicating an important role for soil water in N dynamics at sub-zero soil temperatures. Conclusions: Our findings demonstrate that N uptake by plants and soil N transformation did not cease at sub-zero soil temperatures. Further studies are needed to understand the importance of N dynamics at sub-zero soil temperatures. Springer International Publishing Switzerland, 2015 15 N labeling nationallicence Nitrate nationallicence Sub-zero soil temperature nationallicence Plant nitrogen uptake nationallicence Winter nationallicence Ueda Miki Graduate School of Life Science, Tohoku University, 980-8578, Aoba, Sendai, Japan aut Tokuchi Naoko Field Science Education and Research Center, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut Hiura Tsutom Tomakomai Research Station, Field Science Center for Northern Biosphere, Hokkaido University, 053-0035, Tomakomai, Japan aut Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 205-214 0032-079X 392:1-2<205 2015 392 11104 https://doi.org/10.1007/s11104-015-2453-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2453-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ueda Miki Graduate School of Life Science, Tohoku University, 980-8578, Aoba, Sendai, Japan aut NATIONALLICENCE 700 1- Tokuchi Naoko Field Science Education and Research Center, Kyoto University, Oiwake-cho, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Hiura Tsutom Tomakomai Research Station, Field Science Center for Northern Biosphere, Hokkaido University, 053-0035, Tomakomai, Japan aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 205-214 0032-079X 392:1-2<205 2015 392 11104