caa a22 4500 606217622 CHVBK 20210128101108.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11104-015-2456-y doi (NATIONALLICENCE)springer-10.1007/s11104-015-2456-y Brassicaceae cover crops reduce Aphanomyces pea root rot without suppressing genetic potential of microbial nitrogen cycling [Elektronische Daten] [Shakhawat Hossain, Göran Bergkvist, Robert Glinwood, Kerstin Berglund, Anna Mårtensson, Sara Hallin, Paula Persson] Aims: Brassicaceae cover crops can be used to suppress soil-borne pathogens. The aim was to investigate the effect of different brassicas with different glucosinolate profiles on the development of Aphanomyces pea root rot in subsequent pea plants, and the genetic potential of free-living N2-fixing bacteria and ammonia oxidising bacteria (AOB) and archaea (AOA) performing key soil ecosystem services. Methods: The Brassicaceae species Brassica juncea and Sinapis alba and non-Brassicaceae species Secale cereale were grown for 11-weeks in Aphanomyces euteiches infested soil at low and high nitrogen (N) fertiliser doses. After removing both shoots and roots of the cover crops, peas were grown as a bioassay to evaluate Aphanomyces pea root rot development. Soil was sampled before harvesting the cover crops and at the end of the bioassay. Volatile compounds were collected in the root-soil environment before harvesting the Brassicaceae cover crops to determine the concentration of isothiocyanates. The abundance of genes involved in N2-fixing bacteria and ammonia oxidation in AOA and AOB were assessed. Results: Pea root rot disease severity was reduced in Brassicaceae grown soil at the high N fertiliser dose. This was associated with increased growth of the cover crops. The growth of Brassicaceae did not suppress the abundance of N-cycling microbial communities, but rather increased the AOB at the end of the bioassay, most likely due to increased N availability. The disease suppressive effect was higher with S. alba than with B. juncea, and this coincided with a more diverse composition and higher concentration of aliphatic ITCs released from S. alba roots. Fewer nodules were formed after the Brassicaceae crops, especially Sinapis alba. Conclusions: Brassicaceae cover crops, particularly S. alba, can be used to control soil-borne pathogens without major side effects on the genetic potential of beneficial soil microorganisms involved in N cycling. However, less nodule formation after brassicas indicates an effect on rhizobium activity. The Author(s), 2015 Glucosinolates nationallicence Isothiocyanates nationallicence Aphanomyces euteiches nationallicence Brassica juncea nationallicence Sinapis alba nationallicence Nitrogen fertlisation nationallicence Biofumigation nationallicence Hossain Shakhawat Department of Crop Production Ecology, Swedish University of Agricultural Sciences, P.O. Box 7043, 750 07, Uppsala, Sweden aut Bergkvist Göran Department of Crop Production Ecology, Swedish University of Agricultural Sciences, P.O. Box 7043, 750 07, Uppsala, Sweden aut Glinwood Robert Department of Crop Production Ecology, Swedish University of Agricultural Sciences, P.O. Box 7043, 750 07, Uppsala, Sweden aut Berglund Kerstin Department of Soil and Environment, Swedish University of Agricultural Sciences, P.O. Box 7014, 750 07, Uppsala, Sweden aut Mårtensson Anna Department of Soil and Environment, Swedish University of Agricultural Sciences, P.O. Box 7014, 750 07, Uppsala, Sweden aut Hallin Sara Department of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, 750 07, Uppsala, Sweden aut Persson Paula Department of Crop Production Ecology, Swedish University of Agricultural Sciences, P.O. Box 7043, 750 07, Uppsala, Sweden aut Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 227-238 0032-079X 392:1-2<227 2015 392 11104 https://doi.org/10.1007/s11104-015-2456-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2456-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hossain Shakhawat Department of Crop Production Ecology, Swedish University of Agricultural Sciences, P.O. Box 7043, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Bergkvist Göran Department of Crop Production Ecology, Swedish University of Agricultural Sciences, P.O. Box 7043, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Glinwood Robert Department of Crop Production Ecology, Swedish University of Agricultural Sciences, P.O. Box 7043, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Berglund Kerstin Department of Soil and Environment, Swedish University of Agricultural Sciences, P.O. Box 7014, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Mårtensson Anna Department of Soil and Environment, Swedish University of Agricultural Sciences, P.O. Box 7014, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Hallin Sara Department of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, 750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Persson Paula Department of Crop Production Ecology, Swedish University of Agricultural Sciences, P.O. Box 7043, 750 07, Uppsala, Sweden aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 227-238 0032-079X 392:1-2<227 2015 392 11104