caa a22 4500 606217665 CHVBK 20210128101109.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11104-015-2448-y doi (NATIONALLICENCE)springer-10.1007/s11104-015-2448-y Production of anti-fungal volatiles by non-pathogenic Fusarium oxysporum and its efficacy in suppression of Verticillium wilt of cotton [Elektronische Daten] [Qinghua Zhang, Long Yang, Jing Zhang, Mingde Wu, Weidong Chen, Daohong Jiang, Guoqing Li] Aims: The study aimed at identifying volatile organic compounds (VOCs) produced by the non-pathogenic Fusarium oxysporum (Fo) strain CanR-46, and to determine the anti-fungal spectrum and the control efficacy of the Fo-VOCs. Methods: The Fo-VOCs were identified by GC-MS. The antifungal activity of the Fo-VOCs was tested using the double-dish method. Soil contaminated with Verticillium dahliae was fumigated with the Fo-VOCs to test the control efficacy. The GFP-tagged derivative strain CanR-46GFP was tested to colonize cotton roots for prevention of infection by V. dahliae. Results: Nineteen VOCs were identified with eremophila-1 (10),11-diene being the most abundant. The Fo-VOCs inhibited mycelial growth of 14 fungal species including V. dahliae, delayed V. dahliae conidial germination, suppressed V. dahliae germ-tube elongation, and caused V. dahliae hyphal shriveling and collapse. Four synthetic chemicals, 5-hexenoic acid, limonene, octanoic acid and 3,4-2H-dihydropyran, in the Fo-VOCs profile showed antifungal activity against V. dahliae. The Fo-VOCs significantly (P < 0.05) reduced severity of Verticillium wilt of cotton compared to the control. CanR-46GFP extensively colonized the cotton root tissues, thus effectively prevented V. dahliae infection. Conclusions: The VOC-producing fungus F. oxysporum CanR-46 is a promising biocontrol agent against V. dahliae. Springer International Publishing Switzerland, 2015 Fusarium oxysporum CanR-46 nationallicence Volatile organic compounds nationallicence Verticillium dahliae nationallicence Biological control nationallicence Fumigation nationallicence Zhang Qinghua State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut Yang Long State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut Zhang Jing State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut Wu Mingde State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut Chen Weidong United States Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA, USA aut Jiang Daohong State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut Li Guoqing State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 101-114 0032-079X 392:1-2<101 2015 392 11104 https://doi.org/10.1007/s11104-015-2448-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2448-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Qinghua State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Yang Long State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zhang Jing State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Wu Mingde State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Chen Weidong United States Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA, USA aut NATIONALLICENCE 700 1- Jiang Daohong State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Li Guoqing State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 101-114 0032-079X 392:1-2<101 2015 392 11104