caa a22 4500 606217754 CHVBK 20210128101109.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s11104-015-2457-x doi (NATIONALLICENCE)springer-10.1007/s11104-015-2457-x Elevated ozone increases nitrifying and denitrifying enzyme activities in the rhizosphere of wheat after 5years of fumigation [Elektronische Daten] [Wei Chen, LiLi Zhang, XinYu Li, RongZhong Ye, Qi Li, JianGuo Zhu, NaNa Fang, LingLi Wang, ZhiJie Wu, William Horwath] Backgrounds and aims: Nitrogen (N) cycling in agro-ecosystems is likely to change under elevated O3 (eO3) as a result of changes in plant material inputs to soils. The objective of this study was to understand how eO3 could change key N cycling processes in soils. Methods: Rhizosphere soils were collected from sensitive and tolerant wheat cultivars following a 5-year fumigation of O3 at 60ppb (eO3) and at ambient 40ppb (aO3) in a free-air ozone enrichment platform. Soil nitrifying and denitrifying enzyme activity (NEA and DEA), abundance of ammonia monooxygenase(amoA) and denitrifying bacteria genes(nirS and nirK), and other related properties were quantified. Results: Soil NEA, DEA, NH4 + and NO3 − were higher under eO3 than under aO3, whereas microbial biomass N (MBN) decreased under eO3, especially with the O3-sensitive cultivar. No clear eO3 effect was observed on the abundance of amoA and nirS and nirK. Grain yield and shoot to root ratios decreased as a result of eO3 in the O3-sensitive cultivar. Conclusions: eO3 stimulates soil N availability, but suppresses grain yields after 5years, especially for the O3-sensitive cultivar. Adaptive strategies should focus more on cultivar selection to retain N in soil and to assure the resilience of crop production in the future. Springer International Publishing Switzerland, 2015 Free air ozone enrichment (FAOE) nationallicence Nitrifying enzymes nationallicence Denitrifying enzymes nationallicence Nitrifying genes nationallicence Denitrifying genes nationallicence NEA : Soil nitrifying enzyme activity nationallicence DEA : Soil denitrifying enzyme activity nationallicence Chen Wei Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut Zhang LiLi Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut Li XinYu Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut Ye RongZhong Department of Land Air and Water Resources, University of California, Davis, One shields Avenue, 95616, Davis, CA, USA aut Li Qi Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut Zhu JianGuo State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Fang NaNa Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut Wang LingLi Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut Wu ZhiJie Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut Horwath William Department of Land Air and Water Resources, University of California, Davis, One shields Avenue, 95616, Davis, CA, USA aut Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 279-288 0032-079X 392:1-2<279 2015 392 11104 https://doi.org/10.1007/s11104-015-2457-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-015-2457-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Wei Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Zhang LiLi Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Li XinYu Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Ye RongZhong Department of Land Air and Water Resources, University of California, Davis, One shields Avenue, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Li Qi Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Zhu JianGuo State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Fang NaNa Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Wang LingLi Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Wu ZhiJie Institute of Applied Ecology, Chinese Academy of Sciences, P.O. Box 417, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Horwath William Department of Land Air and Water Resources, University of California, Davis, One shields Avenue, 95616, Davis, CA, USA aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 392/1-2(2015-07-01), 279-288 0032-079X 392:1-2<279 2015 392 11104