caa a22 4500 606217797 CHVBK 20210128101109.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11104-014-2360-x doi (NATIONALLICENCE)springer-10.1007/s11104-014-2360-x Biodegradation of p-hydroxybenzoic acid in soil by Pseudomonas putida CSY-P1 isolated from cucumber rhizosphere soil [Elektronische Daten] [Shu-Yun Chen, Li-Yuan Guo, Ji-Gang Bai, Yue Zhang, Lu Zhang, Zhen Wang, Jin-Xiu Chen, Huan-Xiang Yang, Xiu-Juan Wang] Aims: Accumulation of p-hydroxybenzonic acid (PHBA) in soil will cause plant stress. Our aims were to characterize PHBA-degrading Pseudomonas putida CSY-P1 and to assess its role on alleviating PHBA stress in plants. Methods: Strain CSY-P1 was isolated from rhizosphere soil, and its properties were investigated. The effects of CSY-P1 on soil enzymes and oxidative damage in plants were analyzed. Under PHBA-contaminated environments, antioxidant enzyme activities in CSY-P1 were assessed. Results: Optimal conditions for degradation of PHBA by CSY-P1 were 28°C, pH9, and an initial PHBA concentration of 0.6gl−1. Protocatechoic acid was a metabolite. CSY-P1 decomposed PHBA effectively in unplanted autoclaved soil. After the strain was applied to PHBA-contaminated cucumber-planted soil, activities of some soil enzymes were increased and the PHBA concentration in soil decreased. Some antioxidant enzyme activities in leaves were elevated, thus reducing malondialdehyde levels in seedlings and mitigating PHBA stress in cucumber. Catalase activity in PHBA-exposed CSY-P1 increased. Conclusions: Pseudomonas putida CSY-P1 degrades PHBA in autoclaved soil and alleviates PHBA stress to plants by inducing some soil enzymes and antioxidant enzymes in leaves. Catalase in strain CSY-P1 contributes to its PHBA tolerance, making it a promising strain for remediation of PHBA-contaminated soil. Springer International Publishing Switzerland, 2014 Antioxidant enzyme nationallicence Bioremediation nationallicence P-hydroxybenzonic acid degradation nationallicence Pseudomonas putida nationallicence Soil enzyme nationallicence Chen Shu-Yun State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Guo Li-Yuan State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Bai Ji-Gang State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Zhang Yue State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Zhang Lu State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Wang Zhen State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Chen Jin-Xiu State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Yang Huan-Xiang State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Wang Xiu-Juan State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 197-210 0032-079X 389:1-2<197 2015 389 11104 https://doi.org/10.1007/s11104-014-2360-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2360-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Shu-Yun State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Guo Li-Yuan State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Bai Ji-Gang State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yue State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Lu State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhen State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Chen Jin-Xiu State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Yang Huan-Xiang State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xiu-Juan State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 271018, Tai'an, Shandong, People's Republic of China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 197-210 0032-079X 389:1-2<197 2015 389 11104