caa a22 4500 606217819 CHVBK 20210128101109.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11104-014-2367-3 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2367-3 Root cell wall polysaccharides are involved in cadmium hyperaccumulation in Sedum alfredii [Elektronische Daten] [Tingqiang Li, Qi Tao, Md Shohag, Xiaoe Yang, Donald Sparks, Yongchao Liang] Background and aims: The role of polysaccharide modification in metal accumulation in hyperaccumulators is still unknown. Our aim was to compare the differences in the role of root cell-wall polysaccharides in cadmium (Cd) accumulation between hyperaccumulating (HE) and non-hyperaccumulating ecotype (NHE) of Sedm alfredii. Methods: Hydroponic experiments were performed to characterize root-to-shoot Cd translocation, cadmium species and polysaccharide modification in root cell-wall of S. alfredii using stable isotope tracing, X-ray absorption near edge structure and immunofluorescence localization techniques. Results: Cd absorbed was more readily available for transport to the shoots by the HE roots than by the NHE roots, which is confirmed by a 6-fold higher 113Cd concentration in xylem sap. Root Cd efflux originated mainly from the cell walls. The concentration of cell-wall polysaccharides and activity of pectin methylesterase were higher in the NHE than in the HE in the absence of Cd, and even higher in the presence of Cd. More pectins were methylated in the HE than in the NHE, indicating more free pectic acid residues in the NHE. The cell-wall-bound Cd was retained more tightly in the NHE than in the HE. Conclusions: Cadmium hyperaccumulation by HE of S. alfredii is associated with its enhanced Cd flux into the xylem, which is partly regulated by cell-wall polysaccharide modification in roots. Springer International Publishing Switzerland, 2014 Cadmium nationallicence Cell wall nationallicence Hyperaccumulation nationallicence Pectin nationallicence Polysaccharides nationallicence Sedum alfredii nationallicence Cd : Cadmium nationallicence HC1 : Hemicellulose 1 nationallicence HC2 : Hemicellulose 2 nationallicence HE : Hyperaccumulating ecotype nationallicence ICP-MS : Inductively coupled plasma mass spectrometry nationallicence LCF : Linear-combination fitting nationallicence NHE : Non-hyperaccumulating ecotype nationallicence PME : Pectin methylesterase nationallicence XANES : X-ray absorption near edge structure nationallicence Li Tingqiang Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut Tao Qi Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut Shohag Md Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut Yang Xiaoe Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut Sparks Donald Department of Plant and Soil Sciences, University of Delaware, 19717, Newark, DE, USA aut Liang Yongchao Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 387-399 0032-079X 389:1-2<387 2015 389 11104 https://doi.org/10.1007/s11104-014-2367-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2367-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Tingqiang Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Tao Qi Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Shohag Md Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Yang Xiaoe Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Sparks Donald Department of Plant and Soil Sciences, University of Delaware, 19717, Newark, DE, USA aut NATIONALLICENCE 700 1- Liang Yongchao Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 387-399 0032-079X 389:1-2<387 2015 389 11104