caa a22 4500 606217827 CHVBK 20210128101109.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11104-014-2358-4 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2358-4 Traditional and molecular detection methods reveal intense interguild competition and other multitrophic interactions associated with native entomopathogenic nematodes in Swiss tillage soils [Elektronische Daten] [Raquel Campos-Herrera, Geoffrey Jaffuel, Xavier Chiriboga, Rubén Blanco-Pérez, Marie Fesselet, Vladimír Půža, Fabio Mascher, Ted Turlings] Background and aims: As part of a research consortium that explores ways to improve soil health, we study how entomopathogenic nematodes (EPNs) can be better exploited for the biological control of soil-dwelling insect pests in annual crops. Methods: We evaluated how tillage might affect belowground interactions in two 30-year running Swiss field trials by combining traditional (insect bait) and molecular (novel real-time qPCR protocols) methods. Soil samples (April and October 2013) were evaluated for the presence and activity of EPN soil food web assemblage comprising 13 EPN species, six nematophagous fungi, one ectoparasitic bacterium, and the free-living nematodes (FLN) of the Acrobeloides group. Results: Mortality of sentinel larvae, as well as qPCR analyses (for which we provide seven new primers/probes sets) found only trace levels of six EPN species, dominated by heterorhabditids species. Analysis of nematode progeny revealed that EPN compete intensely with FLN for insect cadavers. Overall, it appears that temperate annual cropping systems provide poor environments for EPN and that tillage does not negatively affect the natural occurrence of EPN. Conclusions: Natural occurrence of EPN in Swiss tillage soils was very low, and augmentation may be a promising strategy to improve the control of root pests of annual crops. Springer International Publishing Switzerland, 2014 Entomopathogenic nematodes nationallicence Annual crops nationallicence Soil food web nationallicence Real-time qPCR nationallicence Galleria mellonella insect-bait nationallicence Campos-Herrera Raquel FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut Jaffuel Geoffrey FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut Chiriboga Xavier FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut Blanco-Pérez Rubén FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut Fesselet Marie Agroscope, Institut of Plant Production Sciences IPS, Route de Duillier 50, CP 1012, 1260, Nyon, Switzerland aut Půža Vladimír Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Mascher Fabio Agroscope, Institut of Plant Production Sciences IPS, Route de Duillier 50, CP 1012, 1260, Nyon, Switzerland aut Turlings Ted FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 237-255 0032-079X 389:1-2<237 2015 389 11104 https://doi.org/10.1007/s11104-014-2358-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2358-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Campos-Herrera Raquel FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut NATIONALLICENCE 700 1- Jaffuel Geoffrey FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut NATIONALLICENCE 700 1- Chiriboga Xavier FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut NATIONALLICENCE 700 1- Blanco-Pérez Rubén FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut NATIONALLICENCE 700 1- Fesselet Marie Agroscope, Institut of Plant Production Sciences IPS, Route de Duillier 50, CP 1012, 1260, Nyon, Switzerland aut NATIONALLICENCE 700 1- Půža Vladimír Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Mascher Fabio Agroscope, Institut of Plant Production Sciences IPS, Route de Duillier 50, CP 1012, 1260, Nyon, Switzerland aut NATIONALLICENCE 700 1- Turlings Ted FARCE Laboratory, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 237-255 0032-079X 389:1-2<237 2015 389 11104