caa a22 4500 606217975 CHVBK 20210128101110.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11104-014-2345-9 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2345-9 Collection and analysis of root exudates of Festuca arundinacea L. and their role in facilitating the phytoremediation of petroleum-contaminated soil [Elektronische Daten] [Wuxing Liu, Jinyu Hou, Qingling Wang, Huijuan Yang, Yongming Luo, Peter Christie] Background and aims: The objectives of this study were to elucidate the mechanisms of interaction between root exudates of tall fescue and functional bacteria associated with petroleum degradation and whether components of the exudates can enhance petroleum removal from soil. Methods: Root exudates of tall fescue were collected through a continuous root exudate trapping system and identified by GC-MS. Chemotaxis, swarming, and in vitro assay were conducted to assess the effects of the organic acids of root exudates on Klebsiella sp. D5A (plant growth promoting rhizobacterium), Pseudomonas sp. SB (biosurfactant producing bacterium), and Streptomyces sp. KT (petroleum-degrading bacterium). A pot experiment with organic acid amendment was conducted to study the effects of these components of root exudates on petroleum remediation. Microbial physiological metabolisms affected by organic acids were tested using Biolog Eco plates. Results: Palmitic acid was found to be most effective in promoting D5A colonization on tall fescue. ρ-Hydroxybenzoic and palmitic acids significantly stimulated the growth of strains D5A, SB, and KT. Furthermore, palmitic acid amendment significantly enhanced petroleum removal in pot experiment. Conclusions: Palmitic acid was the critical organic acid to facilitate petroleum removal during phytoremediation. These findings provide insight into the mechanisms by which tall fescue enhances the degradation of petroleum. Springer International Publishing Switzerland, 2014 Festuca arundinacea L nationallicence Root exudates nationallicence Functional bacteria nationallicence Petroleum phytoremediation nationallicence Liu Wuxing Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Hou Jinyu Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Wang Qingling Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Yang Huijuan Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Luo Yongming Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Christie Peter Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 109-119 0032-079X 389:1-2<109 2015 389 11104 https://doi.org/10.1007/s11104-014-2345-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2345-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wuxing Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Hou Jinyu Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Wang Qingling Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Yang Huijuan Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Luo Yongming Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Christie Peter Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 109-119 0032-079X 389:1-2<109 2015 389 11104