caa a22 4500 606217991 CHVBK 20210128101110.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11104-014-2351-y doi (NATIONALLICENCE)springer-10.1007/s11104-014-2351-y The influence of soils with different textures on development, colonization capacity and interactions between Fusarium culmorum and Pseudomonas fluorescens in soil and on barley roots [Elektronische Daten] [Olga Strunnikova, Nadezhda Vishnevskaya, Anastasia Ruchiy, Vlada Shakhnazarova, Nikolai Vorobyov, Vladimir Chebotar] Backgrounds and aims: Soils are known to influence the survival and the biocontrol activity of Pseudomonas bacteria. However, few studies have focused on the development of the phytopathogenic fungus Fusarium culmorum under various soil conditions and its interactions with the antagonistic microflora. The aims of our research were to study the development of Fusarium culmorum and Pseudomonas fluorescens in two soils with different textures and on barley roots and to assess the influence of these soils in the growth, colonization capacity and interactions between the phytopathogen and the antagonist. Methods: Development of F. culmorum and P. fluorescens was studied in dynamics on membranes in two non-sterile soils with different textures, as well as on barley roots. F. culmorum was identified by indirect immunofluorescent method, and P. fluorescens was visualised with the use of a gus-marked strain. Results: P. fluorescens 2137gus suppressed considerably the mycelium growth and the macroconidia formation of F. culmorum in both soils. However, bacterium did not decrease considerably the density of the fungal mycelium on the surface of barley roots. On the contrary, the amount of the 2137gus was decreased considerably on roots in the light loamy soil in the presence of F. culmorum. Nevertheless, biocontrol effect of P. fluorescens 2137gus was expressed in the light loamy soil. There was no direct correlation between the mycelia density on membranes in soils and the amount of the fungus on roots at the beginning of colonization. The amount of the fungus on root surface did not correlate directly with the intensity of barley root rot. Conclusion: The behaviour and interactions of F. culmorum and P. fluorescens depend on soil type and the habitat (soil or roots). Springer International Publishing Switzerland, 2014 Biocontrol nationallicence Gus-marked strain nationallicence Immunofluorescence nationallicence Root rot nationallicence Strunnikova Olga All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut Vishnevskaya Nadezhda All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut Ruchiy Anastasia All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut Shakhnazarova Vlada All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut Vorobyov Nikolai All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut Chebotar Vladimir All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 131-144 0032-079X 389:1-2<131 2015 389 11104 https://doi.org/10.1007/s11104-014-2351-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2351-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Strunnikova Olga All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut NATIONALLICENCE 700 1- Vishnevskaya Nadezhda All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut NATIONALLICENCE 700 1- Ruchiy Anastasia All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut NATIONALLICENCE 700 1- Shakhnazarova Vlada All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut NATIONALLICENCE 700 1- Vorobyov Nikolai All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut NATIONALLICENCE 700 1- Chebotar Vladimir All-Russia Research Institute for Agriculture Microbiology, Podbelsky shosse, 3, 196608, St. Petersburg-Pushkin, Russia aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 389/1-2(2015-04-01), 131-144 0032-079X 389:1-2<131 2015 389 11104