caa a22 4500 606218092 CHVBK 20210128101111.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11104-014-2326-z doi (NATIONALLICENCE)springer-10.1007/s11104-014-2326-z Colonization of Trichoderma harzianum strain SQR-T037 on tomato roots and its relationship to plant growth, nutrient availability and soil microflora [Elektronische Daten] [Feng Cai, Wei Chen, Zhong Wei, Guan Pang, Ruixia Li, Wei Ran, Qirong Shen] Aims: This research was to study the capacity of Trichoderma harzianum strain SQR-T037 to colonize tomato roots and how this strain and its bio-organic fertilizer stimulate plant growth and increase yields under field conditions. Methods: Field trials were conducted with a reduced application of chemical fertilizer (75% of the recommended application) plus Trichoderma-enriched bio-organic fertilizer (BF) or organic fertilizer (OF) or Trichoderma spore suspension (SS), while 100% of the recommended chemical fertilizer (CF) was used as control. Results: Trichoderma SQR-T037 could efficiently colonize tomato roots and soils based on the reverse transcription-quantitative PCR analysis, and significantly stimulate biomass accumulation at an early stage. The 75% rates of chemical fertilizer coupled with bio-organic fertilizer (BF) produced tomato yields equivalent to those obtained using the 100% rates of chemical fertilizer (CF), while inoculation with the Trichoderma alone (SS) or supplement with organic fertilizer alone (OF) would cause 11 and 13% decreases in yield over the control (CF). The efficacy of BF for maintaining a stable tomato yield may be due to the enhanced soil nutrients availability and the increased abundance of soil microflora, including bacteria, fungi, actinomycetes and Trichoderma communities, which had a positive linear correlation in most of the cases revealed by the Pearson correlation analysis. Conclusions: The results of this study imply that the reason by which the bio-organic fertilizer promotes plant growth and increases the tomato yields can be attributed to the enhancement of the rhizospheric microflora, which promotes nutrient activation. Therefore, T. harzianum could be employed in a combination with composts. In this way, the application rate of chemical fertilizers can be practically decreased by 25% to obtain maximum benefits. Springer International Publishing Switzerland, 2014 Trichoderma harzianum nationallicence Colonization nationallicence Plant growth promotion nationallicence Nutrient availability nationallicence Soil microflora nationallicence Rhizosphere nationallicence PGPM : Plant growth-promoting microbes nationallicence Cfu : Colony forming units nationallicence RT-qPCR : Reverse transcription-quantitative PCR nationallicence PDA : Potato dextrose agar nationallicence AMF : Arbuscular mycorrhiza fungus nationallicence DW : Dry weights nationallicence OM : Organic matter nationallicence ITS : Internal transcribed spacer nationallicence RDA : Redundancy analysis nationallicence Cai Feng Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut Chen Wei Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut Wei Zhong Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut Pang Guan Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut Li Ruixia Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut Ran Wei Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut Shen Qirong National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, 210095, Nanjing, China aut Plant and Soil Springer International Publishing 388/1-2(2015-03-01), 337-350 0032-079X 388:1-2<337 2015 388 11104 https://doi.org/10.1007/s11104-014-2326-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2326-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cai Feng Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Chen Wei Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Wei Zhong Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Pang Guan Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Li Ruixia Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Ran Wei Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Shen Qirong National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 388/1-2(2015-03-01), 337-350 0032-079X 388:1-2<337 2015 388 11104