caa a22 4500 606218157 CHVBK 20210128101111.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11104-014-2321-4 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2321-4 Elemental distribution by cryo-micro-PIXE in the zinc and cadmium hyperaccumulator Sedum plumbizincicola grown naturally [Elektronische Daten] [Pengjie Hu, Yaodong Wang, Wojciech Przybyłowicz, Zhu Li, Alban Barnabas, Longhua Wu, Yongming Luo, Jolanta Mesjasz-Przybyłowicz] Background and aims: The aim of this study was to determine Zn/Cd accumulation ability and cellular distribution in Sedum plumbizincicola from a mine area. Methods: Spatial localization of S, Cl, K, Ca, Ti, Mn, Fe, Ni, Cu, Zn and Cd in frozen-hydrated sections of S. plumbizincicola was quantitatively determined using cryo-micro-PIXE. Results: The shoots of S. plumbizincicola hyperaccumulated up to 1,470mg kg−1 of Cd and 14,600mg kg−1 of Zn (dry weight) in field-grown plants. Micro-PIXE analyse shows that in roots Zn was concentrated in the cortex. In stems Zn was preferentially accumulated in the epidermis (5,090mg kg−1 wet weight) and in vascular bundles and neighbouring parenchyma cells. Interestingly, some vascular bundles of the stem were depleted in Zn. Taking tissue area into account, 22.1, 46.2 and 31.8% of total Zn and 17.1, 71.6 and 11.3% of total Cd in the stem were present in the epidermis, cortex and central cylinder, respectively. In the leaves, 81.3% of total Cd and 55.2% of total Zn were distributed in the mesophyll. Conclusions: The parenchyma cells, e.g. cortex in stem and mesophyll in leaf, play more important roles in Cd storage and detoxification than Zn in S. plumbizincicola. Springer International Publishing Switzerland, 2014 Cellular localization nationallicence Cryo-micro-PIXE nationallicence Elemental mapping nationallicence Frozen-hydrated tissue nationallicence Hyperaccumulation nationallicence X-ray microanalysis nationallicence Hu Pengjie Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Wang Yaodong iThemba LABS, National Research Foundation, PO Box 722, 7129, Somerset West, South Africa aut Przybyłowicz Wojciech iThemba LABS, National Research Foundation, PO Box 722, 7129, Somerset West, South Africa aut Li Zhu Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Barnabas Alban iThemba LABS, National Research Foundation, PO Box 722, 7129, Somerset West, South Africa aut Wu Longhua Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Luo Yongming Yantai Institute of Coastal Zone Research, 264003, Yantai, China aut Mesjasz-Przybyłowicz Jolanta iThemba LABS, National Research Foundation, PO Box 722, 7129, Somerset West, South Africa aut Plant and Soil Springer International Publishing 388/1-2(2015-03-01), 267-282 0032-079X 388:1-2<267 2015 388 11104 https://doi.org/10.1007/s11104-014-2321-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2321-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Pengjie Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Wang Yaodong iThemba LABS, National Research Foundation, PO Box 722, 7129, Somerset West, South Africa aut NATIONALLICENCE 700 1- Przybyłowicz Wojciech iThemba LABS, National Research Foundation, PO Box 722, 7129, Somerset West, South Africa aut NATIONALLICENCE 700 1- Li Zhu Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Barnabas Alban iThemba LABS, National Research Foundation, PO Box 722, 7129, Somerset West, South Africa aut NATIONALLICENCE 700 1- Wu Longhua Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Luo Yongming Yantai Institute of Coastal Zone Research, 264003, Yantai, China aut NATIONALLICENCE 700 1- Mesjasz-Przybyłowicz Jolanta iThemba LABS, National Research Foundation, PO Box 722, 7129, Somerset West, South Africa aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 388/1-2(2015-03-01), 267-282 0032-079X 388:1-2<267 2015 388 11104