caa a22 4500 606218203 CHVBK 20210128101111.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11104-014-2327-y doi (NATIONALLICENCE)springer-10.1007/s11104-014-2327-y The involvement of phenazines and cyclic lipopeptide sessilin in biocontrol of Rhizoctonia root rot on bean ( Phaseolus vulgaris ) by Pseudomonas sp. CMR12a is influenced by substrate composition [Elektronische Daten] [Gia Hua, Monica Höfte] Aims: Pseudomonas sp. CMR12a is an effective biocontrol agent that produces various antifungal metabolites including phenazine antibiotics and cyclic lipopeptides. In this study, we wanted to investigate the influence of substrate composition on the role of phenazines and the cyclic lipopeptide (CLP) sessilin in biocontrol of bean root rot caused by Rhizoctonia solani AG 2-2. Methods: Disease severity and spreading rate of R. solani were determined in three substrates, containing different ratios of potting soil and sand, inoculated with Pseudomonas sp. CMR12a or its mutants impaired in phenazine and/or sessilin biosynthesis. Results: In the mixtures containing 50 or 75% of potting soil, the presence of either phenazines or sessilin was sufficient to suppress bean root rot. However, in the mixture containing only 25% of potting soil, the involvement of both compounds was required to provide better protection. The ratio between potting soil and sand also determined the spreading rate of Rhizoctonia hyphae. The mixture containing 75% of potting soil was the most suppressive against R. solani invasion. Conclusions: The use of various potting soil/sand combinations affected the development of bean root rot disease but Pseudomonas sp. CMR12a effectively controlled the disease in all substrates. Depending on the disease pressure, however, the production of either phenazines or sessilin, or both compounds is required for effective biocontrol. Springer International Publishing Switzerland, 2014 Biocontrol nationallicence Cyclic lipopeptides nationallicence Phenazines nationallicence Sessilin nationallicence Fluorescent pseudomonads nationallicence Rhizoctonia solani nationallicence Hua Gia Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Höfte Monica Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Plant and Soil Springer International Publishing 388/1-2(2015-03-01), 243-253 0032-079X 388:1-2<243 2015 388 11104 https://doi.org/10.1007/s11104-014-2327-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2327-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hua Gia Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Höfte Monica Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 388/1-2(2015-03-01), 243-253 0032-079X 388:1-2<243 2015 388 11104