caa a22 4500 606218343 CHVBK 20210128101112.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11104-014-2325-0 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2325-0 Possible involvement of heterotrimeric G-protein signaling in Al-induced secretion of organic acid anions in Arabidopsis and rye [Elektronische Daten] [Yao Li, Xin Tang, Lei Yang, Yong Yu, Xiao Li] Background and aims: Secretion of organic acid anions (OAs) from the roots in response to aluminum (Al) is a common mechanism for Al resistance in plants. Transporter genes involved in the Al-induced secretion of malate and citrate have been identified, but the signal cascades mediating the regulation of the secretion of OAs has not been understood. Here, we investigated the possible role of heterotrimeric G-proteins in the Al-induced secretion of OAs. Methods: Seedlings of rye and Arabidopsis thaliana were grown in an environment-controlled growth room. Organic acid anions in the exudates of roots were determined spectrophotometrically. Heterotrimeric G-proteins and GTPase activity were assayed using protein gel blot analyses with Gα antibody and spectrofluorometer, respectively. GPA1 gene expression level was determined by semi-quantitative RT-PCR. Results: Immunoblotting revealed a heterotrimeric G-protein in the plasma membrane fraction of rye and Arabidopsis roots. Exposure of the rye roots to Al resulted in activation of GTPase. Al-induced secretion was inhibited by pertussis toxin, a heterotrimeric G-protein antagonist, but was stimulated by cholera toxin, a heterotrimeric G-protein agonist. Furthermore, Al-induced OA secretion was blocked in Gα- or Gβ-deficient mutants of Arabidopsis. Al induced GPA1 expression in Arabidopsis roots. Conclusions: Heterotrimeric G-proteins-coordinated transduction of Al-stress signals may be associated with the secretion of OAs from roots. Springer International Publishing Switzerland, 2014 Heterotrimeric G-protein nationallicence Secretion nationallicence Organic acids nationallicence Al nationallicence Signaling nationallicence Li Yao State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, 530005, Nanning, China aut Tang Xin State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, 530005, Nanning, China aut Yang Lei State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, 530005, Nanning, China aut Yu Yong College of Animal Science and Technology, Southwest University, 400716, Beibei Chongqing, China aut Li Xiao State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, 530005, Nanning, China aut Plant and Soil Springer International Publishing 388/1-2(2015-03-01), 55-63 0032-079X 388:1-2<55 2015 388 11104 https://doi.org/10.1007/s11104-014-2325-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2325-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Yao State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, 530005, Nanning, China aut NATIONALLICENCE 700 1- Tang Xin State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, 530005, Nanning, China aut NATIONALLICENCE 700 1- Yang Lei State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, 530005, Nanning, China aut NATIONALLICENCE 700 1- Yu Yong College of Animal Science and Technology, Southwest University, 400716, Beibei Chongqing, China aut NATIONALLICENCE 700 1- Li Xiao State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, 530005, Nanning, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 388/1-2(2015-03-01), 55-63 0032-079X 388:1-2<55 2015 388 11104