caa a22 4500 60621836X CHVBK 20210128101112.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11104-014-2277-4 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2277-4 Soil C:N ratio is the major determinant of soil microbial community structure in subtropical coniferous and broadleaf forest plantations [Elektronische Daten] [Xiaohua Wan, Zhiqun Huang, Zongming He, Zaipeng Yu, Minhuang Wang, Murray Davis, Yusheng Yang] Aims: This study aimed to determine the influence of tree species on soil microbial community structure. Methods: We conducted a litter and root manipulation and a short-term nitrogen (N) addition experiment in 19-year-old broadleaf Mytilaria laosensis (Hamamelidaceae) and coniferous Chinese fir (Cunninghamia lanceolata) plantations in subtropical China. Phospholipid fatty acid (PLFA) analysis was used to examine treatment effects on soil microbial community structure. Redundancy analysis (RDA) was performed to determine the relationships between individual PLFAs and soil properties (soil pH, carbon (C) and N concentration and C:N ratio). Results: Soil C:N ratio was significantly greater in M. laosensis (17.9) than in C. lanceolata (16.2). Soil C:N ratio was the key factor affecting the soil microbial community regardless of tree species and the litter, root and N treatments at our study site. The fungal biomarkers, 18:1ω9 and 18:2ω6,9 were significantly and positively related to soil C:N ratio and the abundance of bacterial lipid biomarkers was negatively related to soil C:N ratio. N addition for 8months did not change the biomass and structure of the microbial community in M. laosensis and C. lanceolata soils. Soil nutrient availability before N addition was an important factor in determining the effect of N fertilization on soil microbial biomass and activity. PLFA analysis showed that root exclusion significantly decreased the abundance of the fungal biomarkers and increased the abundance of the Gram-positive bacteria. Rootless plots had a relatively lower Gram-positive to Gram-negative bacteria ratio and a higher fungi to bacteria ratio compared to the plots with roots under both M. laosensis and C. lanceolata. The response of arbuscular mycorrhizal fungi (16:1ω5) to root exclusion was species-specific. Conclusions: These observations suggest that soil C:N ratio was an important factor in influencing soil microbial community structure. Further studies are required to confirm the long-term effect of tree species on soil microbial community structure. Springer International Publishing Switzerland, 2014 N addition nationallicence Phospholipid fatty acids (PLFA) nationallicence Root exclusion nationallicence Soil properties nationallicence Tree species nationallicence Wan Xiaohua College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut Huang Zhiqun College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut He Zongming College of Forestry, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Yu Zaipeng College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut Wang Minhuang College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut Davis Murray Scion, P.O. Box 29237, 8540, Christchurch, Fendalton, New Zealand aut Yang Yusheng College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 103-116 0032-079X 387:1-2<103 2015 387 11104 https://doi.org/10.1007/s11104-014-2277-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2277-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wan Xiaohua College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut NATIONALLICENCE 700 1- Huang Zhiqun College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut NATIONALLICENCE 700 1- He Zongming College of Forestry, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Yu Zaipeng College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut NATIONALLICENCE 700 1- Wang Minhuang College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut NATIONALLICENCE 700 1- Davis Murray Scion, P.O. Box 29237, 8540, Christchurch, Fendalton, New Zealand aut NATIONALLICENCE 700 1- Yang Yusheng College of Geographical Science, Fujian Normal University, 350007, Fuzhou, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 103-116 0032-079X 387:1-2<103 2015 387 11104