caa a22 4500 606218416 CHVBK 20210128101112.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11104-014-2295-2 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2295-2 Differential plant growth promotion and nitrogen fixation in two genotypes of maize by several Herbaspirillum inoculants [Elektronische Daten] [Gabriela Alves, Sandy Videira, Segundo Urquiaga, Veronica Reis] Aims: Herbaspirillum spp. has been described as bacteria that colonizes not only maize roots but also stems and leaves. This study aimed to access the growth-promoting effect of 21 strains of Herbaspirillum on maize plants. This study is composed by 12 strains of the H. seropedicae, 5 strains of the H. rubrisubalbicans and 4 strains of the H. frisingense. Methods: Bacterial selection was performed in two phases, consisting on planting the BRS4157 maize variety and hybrid SHS5050 in the greenhouse, with sterile and non-sterile substrate and in the field. After 40days, the greenhouse plants were harvested and the dry matter accumulation of the aerial part and roots were measured. The best-performing strain was tested in field trials treated with 40 and 80kgha−1 of N fertilizer, parallel to the same treatments without inoculation. In this trial, the grain yield was evaluated as well as the contribution of biological nitrogen fixation (BNF), using the technique of natural abundance of 15 N. Results: The results showed that H. seropedicae ZAE94 was the best strain under controlled conditions and its application as a field inoculant increased maize yield up to 34%, depending on the plant genotype. The BNF quantification revealed that 37% BNF-derived plant nitrogen in the hybrid SHS5050 inoculated with the H. seropedicae strain ZAE94. Conclusions: The difference in response to nitrogen as well as to inoculation depends on the selected plant genotype and the bacterial inoculant. H. seropedicae ZAE94 can promote plant growth and contribute biologically with fixed nitrogen in the maize hybrid SHS5050. Springer International Publishing Switzerland, 2014 Nitrogen nationallicence Biological nitrogen fixation nationallicence Zea mays nationallicence Endophytic bacterium nationallicence Alves Gabriela Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, km 07, BR 465, CEP 23890-000, Seropédica, Brazil aut Videira Sandy Centro Universitário de Volta Redonda (UniFOA), CEP: 27240-560, Volta Redonda-RJ, Brazil aut Urquiaga Segundo Embrapa Agrobiologia, km 07 BR 465, CEP 23891-000, Seropédica, RJ, Brazil aut Reis Veronica Embrapa Agrobiologia, km 07 BR 465, CEP 23891-000, Seropédica, RJ, Brazil aut Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 307-321 0032-079X 387:1-2<307 2015 387 11104 https://doi.org/10.1007/s11104-014-2295-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2295-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alves Gabriela Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, km 07, BR 465, CEP 23890-000, Seropédica, Brazil aut NATIONALLICENCE 700 1- Videira Sandy Centro Universitário de Volta Redonda (UniFOA), CEP: 27240-560, Volta Redonda-RJ, Brazil aut NATIONALLICENCE 700 1- Urquiaga Segundo Embrapa Agrobiologia, km 07 BR 465, CEP 23891-000, Seropédica, RJ, Brazil aut NATIONALLICENCE 700 1- Reis Veronica Embrapa Agrobiologia, km 07 BR 465, CEP 23891-000, Seropédica, RJ, Brazil aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 307-321 0032-079X 387:1-2<307 2015 387 11104