caa a22 4500 606218424 CHVBK 20210128101112.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11104-014-2293-4 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2293-4 Proteomic characterization of silicon-mediated resistance against Ralstonia solanacearum in tomato [Elektronische Daten] [Yuting Chen, Min Liu, Lei Wang, Weipeng Lin, Xueying Fan, Kunzheng Cai] Background and aims: Silicon has an important role in enhancing resistance of plants against multiple environmental stresses including pathogen infection, but the mechanism is still not completely understood. In the present study, the role of Si-mediated resistance to Ralstonia solanacearum in tomato (Solanum lycopersicum) root was explored by a proteomics approach. Methods: Treatments consisted of pathogen inoculation with or without 2.0mM Si amendments, and two controls with or without 2.0mM Si amendments. Proteins from the tomato roots with different treatments were extracted and identified by two-dimensional gel electrophoresis (2-DE) and liquid chromatography-mass spectrometry (LC-MS/MS). Results: Fifty-three protein spots were identified at least two-fold differences in abundance on 2-DE maps under R. solanacearum inoculation and/or Si application. Among these proteins, 40 were significantly altered (6 were up-accumulated and 34 were down-accumulated) by R. solanacearum inoculation only. And 26 were altered (16 were increased and 10 were decreased) when Si was added to R. solanacearum-inoculated tomato plants. More than half of the altered proteins (62%) were associated with energy/metabolism including glycolytic pathway and TCA cycle. Five proteins were grouped into defense-response, of which four were membrane-associated proteins. Conclusions: These findings provide insights into molecular mechanisms responsible for Si-mediated resistance of tomato against R. solanacearum. Springer International Publishing Switzerland, 2014 Silicon nationallicence Tomato nationallicence Ralstonia solanacearum nationallicence 2-DE nationallicence Proteomics nationallicence Induced resistance nationallicence 2-DE : Two-dimensional electrophoresis nationallicence 2-D IEF/SDS-PAGE : 2 dimensional isoelectric focusing/sodium dodecyl sulphate-polyacrylamide gel electrophoresis nationallicence TTC : 2,3,5-Triphenyltetrazolium chloride nationallicence DMRT : Duncan multiple range test nationallicence IEF : Isoelectric focusing nationallicence Si : Silicon nationallicence R. solanacearum : Ralstonia solanacearum nationallicence IPG : Immobilized pH gradient nationallicence SDS-PAGE : Sodium dodecyl sulfate polyacrylamide gel electrophoresis nationallicence MS : Mass spectrometry nationallicence CBB : Coomassie Brilliant Blue nationallicence Chen Yuting Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut Liu Min Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut Wang Lei Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut Lin Weipeng Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut Fan Xueying Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut Cai Kunzheng Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 425-440 0032-079X 387:1-2<425 2015 387 11104 https://doi.org/10.1007/s11104-014-2293-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2293-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Yuting Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Liu Min Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Lei Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Lin Weipeng Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Fan Xueying Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Cai Kunzheng Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, 510642, Guangzhou, China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 425-440 0032-079X 387:1-2<425 2015 387 11104