caa a22 4500 606218459 CHVBK 20210128101112.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11104-014-2290-7 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2290-7 Transcriptional and physiological analyses identify a regulatory role for hydrogen peroxide in the lignin biosynthesis of copper-stressed rice roots [Elektronische Daten] [Qingquan Liu, Li Zheng, Fei He, Fang-Jie Zhao, Zhenguo Shen, Luqing Zheng] Aims: Induction of lignin biosynthesis is an adaptive response of plants subjected to many abiotic stresses. In this study, we examined the response of lignin biosynthesis to copper (Cu) stress, with a particular focus on the regulatory mechanism. Methods: We performed a transcriptomic analysis of rice (Oryza sativa L.) roots, and the microarray data on lignin biosynthesis pathway genes were corroborated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. Physiological analyses of rice seedlings treated with Cu(II) sulfate (CuSO4) were used to confirm the relationship between excess Cu and lignin biosynthesis. In addition, we examined the role of hydrogen peroxide (H2O2) in Cu-induced lignin biosynthesis through pretreatments with an NADPH oxidase inhibitor (diphenyleneiodonium, DPI) and a H2O2 scavenger (dimethylthiourea, DMTU). Results: Lignin biosynthesis pathway genes were upregulated under Cu stress. The lignin content of rice roots increased significantly with increasing concentrations and durations of Cu treatment; elevations in root lignin content were correlated with marked inhibitions in root growth. Pretreatments with DPI and DMTU inhibited the activities of Cu-induced lignin polymerization enzymes (peroxidase, POD and laccase, LAC) and lignin accumulation in rice roots. Conversely, exogenous H2O2 increased the root lignin content. Conclusions: Rice roots under Cu stress accumulate lignin through enhanced polymerization of lignin monolignol, a mechanism that requires Cu stress induced H2O2. Springer International Publishing Switzerland, 2014 Copper nationallicence Oryza sativa L nationallicence Lignin biosynthesis nationallicence Peroxidase nationallicence Laccase nationallicence Liu Qingquan College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Zheng Li College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut He Fei Genenergy company, 200031, Shanghai, People's Republic of China aut Zhao Fang-Jie State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Shen Zhenguo College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Zheng Luqing College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 323-336 0032-079X 387:1-2<323 2015 387 11104 https://doi.org/10.1007/s11104-014-2290-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2290-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Qingquan College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Li College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- He Fei Genenergy company, 200031, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Fang-Jie State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Shen Zhenguo College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Luqing College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 323-336 0032-079X 387:1-2<323 2015 387 11104