caa a22 4500 606218548 CHVBK 20210128101113.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11104-014-2283-6 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2283-6 The importance of a sterile rhizosphere when phenotyping for root exudation [Elektronische Daten] [René Kuijken, Jan Snel, Martijn Heddes, Harro Bouwmeester, Leo Marcelis] Background and aims: A fast and reliable phenotyping system forms a major bottleneck in root exudation research. Our goal was to develop such a system to quantify genetic and environmental effects on root exudation. Another aim was characterizing non-sterile phenotyping. Methods: We developed a system in which plants can be grown with non-sterile shoot environments and sterile rhizospheres. These sterile systems were compared with non-sterile controls. Results: In non-sterile rhizospheres exogenous carbon disappeared quickly with a half-life of 2 to 3h and root exudate concentrations remained below detection limit. In sterile rhizospheres exogenous carbon levels were relatively stable or depleted slower than in non-sterile rhizospheres and organic acid build-up occurred. Tomato (Solanum lycopersicum) could be grown with sterile roots for several months. Conclusions: The differences in carbon depletion in sterile and non-sterile rhizospheres was most likely due to the absence of microbial catabolism in sterile rhizospheres. These results prove that using a sterile phenotyping system is essential to study the quantity and composition of root exudates. The sterile system described in this paper eliminates the obscuring effect caused by microbes on exudation levels. It offers a stable, reliable and easy phenotyping method and can be used to investigate genetic and environmental effects on exudation. Springer International Publishing Switzerland, 2014 Organic acids nationallicence Phenotyping nationallicence Rhizodeposition nationallicence Rhizosphere nationallicence Tomato nationallicence Root exudation nationallicence Kuijken René Wageningen University and Research Centre, Greenhouse Horticulture, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut Snel Jan Wageningen University and Research Centre, Greenhouse Horticulture, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut Heddes Martijn Wageningen University and Research Centre, Greenhouse Horticulture, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut Bouwmeester Harro Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut Marcelis Leo Horticulture and Product Physiology, Wageningen University, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 131-142 0032-079X 387:1-2<131 2015 387 11104 https://doi.org/10.1007/s11104-014-2283-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2283-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuijken René Wageningen University and Research Centre, Greenhouse Horticulture, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Snel Jan Wageningen University and Research Centre, Greenhouse Horticulture, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Heddes Martijn Wageningen University and Research Centre, Greenhouse Horticulture, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Bouwmeester Harro Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Marcelis Leo Horticulture and Product Physiology, Wageningen University, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 131-142 0032-079X 387:1-2<131 2015 387 11104