caa a22 4500 606218572 CHVBK 20210128101113.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11104-014-2297-0 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2297-0 In planta biocontrol of soilborne Fusarium wilt of banana through a plant endophytic bacterium, Burkholderia cenocepacia 869T2 [Elektronische Daten] [Ying-Ning Ho, Hsing-Mei Chiang, Chih-Ping Chao, Ching-Chung Su, Hui-Fang Hsu, Chen-tong Guo, Ju-Liang Hsieh, Chieh-Chen Huang] Aim: Fusarium wilt (Panama disease) caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is a soilborne disease that severely devastates the banana industry worldwide. We aimed to isolate beneficial endophytic bacterial strains against Panama disease. Methods: From different plant species, including reeds (Phragmites australis), vetiver grass (Chrysopogon zizanioides), and banana plants (Cavendish cv. Pei-Chiao, Cavendish cv. Formosana, and Musa sapientum cv. Rose), endophytes were screened and characterized. The diversity and community of endophytes within banana plants were analyzed by PCR-denaturing gradient gel electrophoresis (DGGE). The banana tissue culture plantlets were inoculated with the candidate endophyte, Burkholderia cenocepacia 869T2, and effects of in planta biocontrol were observed. Results: Endophytic B. cenocepacia 869T2 decreased the disease incidence of Fusarium wilt on treated banana plants to 3.4%, comparing to 24.5% of non-inoculated plants infected in the field test within a 7-month period. Furthermore, significant growth promoting of 869T2 inoculated banana plants was observed in field experiments. Conclusions: In addition to 869T2 genomic sequence data, our results suggest that the pyrrolnitrin and pyrroloquinoline quinone potential producer, B. cenocepacia 869T2, is a good biological control agent (BCA) for use in the biocontrol of Fusarium wilt and plant promotion. Springer International Publishing Switzerland, 2014 Banana nationallicence Endophytic bacterium nationallicence Burkholderia cenocepacia 869T2 nationallicence Biocontrol nationallicence Fusarium wilt nationallicence Ho Ying-Ning Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut Chiang Hsing-Mei Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut Chao Chih-Ping Taiwan Banana Research Institute, No.1, Rongquan St., Jiuru Township, Pingtung County, 904, Taiwan, Republic of China aut Su Ching-Chung Taiwan Banana Research Institute, No.1, Rongquan St., Jiuru Township, Pingtung County, 904, Taiwan, Republic of China aut Hsu Hui-Fang Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut Guo Chen-tong Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut Hsieh Ju-Liang Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut Huang Chieh-Chen Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 295-306 0032-079X 387:1-2<295 2015 387 11104 https://doi.org/10.1007/s11104-014-2297-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2297-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ho Ying-Ning Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Chiang Hsing-Mei Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Chao Chih-Ping Taiwan Banana Research Institute, No.1, Rongquan St., Jiuru Township, Pingtung County, 904, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Su Ching-Chung Taiwan Banana Research Institute, No.1, Rongquan St., Jiuru Township, Pingtung County, 904, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Hsu Hui-Fang Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Guo Chen-tong Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Hsieh Ju-Liang Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Huang Chieh-Chen Department of Life Sciences, College of Life Sciences, National Chung Hsing University, 250, Kuo Kuang Rd., 402, Taichung, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 295-306 0032-079X 387:1-2<295 2015 387 11104