caa a22 4500 606218629 CHVBK 20210128101113.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11104-014-2279-2 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2279-2 Root transcriptome analysis on the grape genotypes with contrast translocation pattern of excess manganese from root to shoot [Elektronische Daten] [Yinan Yao, Xinlong Xiao, Yongbin Ou, Xiuli Wu, Gang Xu] Aims: The root-to-shoot transport of manganese (Mn) exhibited intra-specific characters in different grape genotypes. The majority of Mn was stored in the roots of the grape cultivar Jinshou, while it was mainly transferred to the shoots in the cultivar Combier. The aims of the present study was to reveal the complex interplay of gene expression endowing grape a high tolerance to excess Mn and to explore the relation of the expression of Mn transporters with the contrast root-to-shoot translocation pattern of excess Mn in different grape cultivars. Methods: The root transcriptome changes in both cultivars were analyzed by high-throughput sequencing and validated by quantitative RT-PCR. Results and conclusion: Compared to Jinshou, Combier exhibited a markedly high transcripts level in the Mn transporter unigenes in the roots independent of the Mn treatment, accompanied by a higher expression level of genes encoding nicotianamine synthase, heavy metal-transporting ATPase, ZIP family member and IRT1-like proteins, which could facilitate Mn transport from the roots to the shoots in Combier. However, the expression level of genes involved in the subcellular vesicular transport pathway was much higher in Jinshou than in Combier, with a higher transcripts level of V-ATPase, vacuolar protein and the proteins for the synthesis of organic acid, such as the citrate cycle and glycolysis pathway. All of these changes allowed Mn to be easily chelated and compartmented to root cortical and epidermic cell vacuoles in Jinshou, accompanied with higher transcription and activity levels of stress-related enzymes, endowing Jinshou a high degree of tolerance to excess Mn. The grape transcriptome responses to Mn stress were also discussed. Springer International Publishing Switzerland, 2014 Excess manganese nationallicence Transcriptome nationallicence Manganese transporter nationallicence Chelator nationallicence Subcellular vesicular transport nationallicence Aquaporins nationallicence Mn : Manganese nationallicence DEG : Differentially expressed gene nationallicence DGE : Digital gene expression nationallicence TPM : Number of transcripts per million clean tags nationallicence PCR : Polymerase chain reaction nationallicence qRT-PCR : Quantitative real time polymerase chain reaction nationallicence CAT : Catalase nationallicence APX : Ascorbate peroxidase nationallicence POD : Guaiacol peroxidase nationallicence SOD : Superoxide dismutase nationallicence CK : The control nationallicence MDA : Malondialdehyde nationallicence BTC : Biological transfer coefficient nationallicence KEGG : Kyoto Encyclopedia of Genes and Genomes nationallicence IRT : Iron-regulated transporter nationallicence MT : Metallothioneins nationallicence HMA : Heavy metal-transporting P-type ATPase nationallicence NAS : Nicotianamine synthase nationallicence MTP : Metal tolerance protein nationallicence Yao Yinan School of life science and engineering, Southwest University of Science and Technology, 621010, Mianyang, China aut Xiao Xinlong Key Laboratory of Biogeography and Bioresources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, 830011, Urumqi, China aut Ou Yongbin School of life science and engineering, Southwest University of Science and Technology, 621010, Mianyang, China aut Wu Xiuli School of life science and engineering, Southwest University of Science and Technology, 621010, Mianyang, China aut Xu Gang Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals of Guizhou University, Guizhou University, 550025, Guiyang, People's Republic of China aut Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 49-67 0032-079X 387:1-2<49 2015 387 11104 https://doi.org/10.1007/s11104-014-2279-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2279-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yao Yinan School of life science and engineering, Southwest University of Science and Technology, 621010, Mianyang, China aut NATIONALLICENCE 700 1- Xiao Xinlong Key Laboratory of Biogeography and Bioresources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, 830011, Urumqi, China aut NATIONALLICENCE 700 1- Ou Yongbin School of life science and engineering, Southwest University of Science and Technology, 621010, Mianyang, China aut NATIONALLICENCE 700 1- Wu Xiuli School of life science and engineering, Southwest University of Science and Technology, 621010, Mianyang, China aut NATIONALLICENCE 700 1- Xu Gang Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals of Guizhou University, Guizhou University, 550025, Guiyang, People's Republic of China aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 49-67 0032-079X 387:1-2<49 2015 387 11104