caa a22 4500 606218653 CHVBK 20210128101114.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11104-014-2288-1 doi (NATIONALLICENCE)springer-10.1007/s11104-014-2288-1 Arbuscular mycorrhizal fungal communities are influenced by agricultural land use and not soil type among the Chernozem great groups of the Canadian Prairies [Elektronische Daten] [Luke Bainard, Mulan Dai, Eduardo Gomez, Yamir Torres-Arias, Jillian Bainard, Min Sheng, Warren Eilers, Chantal Hamel] Background and aims: The aim of this study was to explore the diversity of arbuscular mycorrhizal (AM) fungi in agroecosystems of the Canadian Prairies and determine whether soil type and land use impact the diversity and composition of these important communities. Methods: We used two separate field surveys and methods to address these questions. The first survey involved collecting soil samples from five different soil types and spores were morphologically identified from trap cultures established using these soil samples. The second survey involved collecting soil samples from two different land use types across the four major Chernozem great groups to characterize the AM fungal communities using 454 GS FLX pyrosequencing of the small subunit rDNA region. Results: The first survey found that Vertisolic soil had a significantly higher AM spore richness compared to the Chernozem soils. Both surveys revealed no effect of soil type on the diversity and composition of AM fungal communities among the Chernozem great groups. The two different methods used in this study revealed differences in the proportional representation of certain AM fungal taxa. Land use had a strong impact on the AM fungal communities in the Canadian Prairies as roadsides harboured a more diverse and compositionally different communities compared to annually cropped fields. Conclusions: Overall, soil type appears to have little to no effect on AM fungal communities among the Chernozem great groups in the Canadian Prairies, and land use practices impact the diversity and composition of these communities. Her Majesty the Queen in Right of Canada, 2014 454 pyrosequencing nationallicence Arbuscular mycorrhizal fungi nationallicence Agriculture nationallicence Soil type nationallicence Community composition nationallicence Diversity nationallicence Land use nationallicence Bainard Luke Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, S9H 3X2, Swift Current, SK, Canada aut Dai Mulan Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, S9H 3X2, Swift Current, SK, Canada aut Gomez Eduardo Instituto de Ecología y Sistemática, Carretera Varona 11835 e/ Oriente y Lindero, La Habana 19, Calabazar, Boyeros, CP 11900, La Habana, Cuba aut Torres-Arias Yamir Instituto de Ecología y Sistemática, Carretera Varona 11835 e/ Oriente y Lindero, La Habana 19, Calabazar, Boyeros, CP 11900, La Habana, Cuba aut Bainard Jillian Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, S7N 5A8, Saskatoon, SK, Canada aut Sheng Min Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, S9H 3X2, Swift Current, SK, Canada aut Eilers Warren Retired, Saskatoon Research Centre, Agriculture and Agri-Food Canada (AAFC), 107 Science Place, S7N 0X2, Saskatoon, SK, Canada aut Hamel Chantal Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, S9H 3X2, Swift Current, SK, Canada aut Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 351-362 0032-079X 387:1-2<351 2015 387 11104 https://doi.org/10.1007/s11104-014-2288-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11104-014-2288-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bainard Luke Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, S9H 3X2, Swift Current, SK, Canada aut NATIONALLICENCE 700 1- Dai Mulan Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, S9H 3X2, Swift Current, SK, Canada aut NATIONALLICENCE 700 1- Gomez Eduardo Instituto de Ecología y Sistemática, Carretera Varona 11835 e/ Oriente y Lindero, La Habana 19, Calabazar, Boyeros, CP 11900, La Habana, Cuba aut NATIONALLICENCE 700 1- Torres-Arias Yamir Instituto de Ecología y Sistemática, Carretera Varona 11835 e/ Oriente y Lindero, La Habana 19, Calabazar, Boyeros, CP 11900, La Habana, Cuba aut NATIONALLICENCE 700 1- Bainard Jillian Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, S7N 5A8, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Sheng Min Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, S9H 3X2, Swift Current, SK, Canada aut NATIONALLICENCE 700 1- Eilers Warren Retired, Saskatoon Research Centre, Agriculture and Agri-Food Canada (AAFC), 107 Science Place, S7N 0X2, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Hamel Chantal Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food Canada (AAFC), 1 Airport Road, S9H 3X2, Swift Current, SK, Canada aut NATIONALLICENCE 773 0- Plant and Soil Springer International Publishing 387/1-2(2015-02-01), 351-362 0032-079X 387:1-2<351 2015 387 11104